Oldenburg Amy L, Chhetri Raghav K, Hill David B, Button Brian
Department of Physics and Astronomy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA ; Biomedical Research Imaging Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Biomed Opt Express. 2012 Sep 1;3(9):1978-92. doi: 10.1364/BOE.3.001978. Epub 2012 Aug 1.
Muco-ciliary transport in the human airway is a crucial defense mechanism for removing inhaled pathogens. Optical coherence tomography (OCT) is well-suited to monitor functional dynamics of cilia and mucus on the airway epithelium. Here we demonstrate several OCT-based methods upon an actively transporting in vitro bronchial epithelial model and ex vivo mouse trachea. We show quantitative flow imaging of optically turbid mucus, semi-quantitative analysis of the ciliary beat frequency, and functional imaging of the periciliary layer. These may translate to clinical methods for endoscopic monitoring of muco-ciliary transport in diseases such as cystic fibrosis and chronic obstructive pulmonary disease (COPD).
人类气道中的黏液纤毛运输是清除吸入病原体的关键防御机制。光学相干断层扫描（OCT）非常适合监测气道上皮细胞上纤毛和黏液的功能动态。在此，我们在一个主动运输的体外支气管上皮模型和离体小鼠气管上展示了几种基于OCT的方法。我们展示了光学浑浊黏液的定量流动成像、纤毛摆动频率的半定量分析以及纤毛周层的功能成像。这些方法可能转化为用于内窥镜监测囊性纤维化和慢性阻塞性肺疾病（COPD）等疾病中黏液纤毛运输的临床方法。
