School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, Jiangsu, China.
Molecules. 2012 Oct 1;17(10):11669-79. doi: 10.3390/molecules171011669.
Active ingredients in different lengths of black soybean sprouts were extracted with water. Concentrations of the main proteins and polysaccharides were determined by the Forint phenol assay and phenol-sulfuric acid assay, respectively. Anti-oxidizing capacities of the extracts were measured in vitro using the DPPH scavenging test and whitening capacity was measured in vitro using the tyrosinase inhibition test. The effects of the bean sprout extracts on human skin fibroblasts damnified by H₂O₂ were studied using an MTT colorimetric assay. The safety of the extracts was determined using the red blood cell (RBC) test, chick chorioallantoic membrane (CAM) assay and human patch test. Results show that DPPH radical scavenging rates at different shoot lengths were all greater than 95%, while the tyrosinase inhibition capacity of the extracts reached 98%. Hemolysis rate in all extracts were lower than 10%, below the 20% regulatory limit for the RBC test. No signs of allergic reactions were observed in the human patch tests. The optimum extract was obtained from bean sprouts grown to 0.5 cm. Extracts of black bean sprouts are safe and can be used as additives in anti-aging and whitening cosmetic products.
将不同长度的黑豆芽用水分提取其中的活性成分。通过福林酚比色法和苯酚-硫酸法分别测定主要蛋白质和多糖的浓度。采用 DPPH 清除试验体外测定提取物的抗氧化能力，采用酪氨酸酶抑制试验体外测定其美白能力。通过 MTT 比色法研究了豆芽提取物对 H₂O₂损伤的人皮肤成纤维细胞的作用。通过红细胞（RBC）试验、鸡胚绒毛尿囊膜（CAM）试验和人体斑贴试验测定提取物的安全性。结果表明，不同芽长的 DPPH 自由基清除率均大于 95%，而提取物对酪氨酸酶的抑制能力达到 98%。所有提取物的溶血率均低于 10%，低于 RBC 试验 20%的规定限值。人体斑贴试验未观察到过敏反应迹象。最佳提取物来自生长至 0.5 厘米的豆芽。黑豆芽提取物安全，可作为抗衰老和美白化妆品的添加剂。
