Espevik T, Waage A, Faxvaag A, Shalaby M R
Institute of Cancer Research, University of Trondheim, Norway.
Cell Immunol. 1990 Mar;126(1):47-56. doi: 10.1016/0008-8749(90)90299-7.
The effect of recombinant (r) interleukin-1 beta (rIL-1 beta) and transforming growth factor-beta (TGF-beta) on the production of interleukin-2 (IL-2) and interleukin-6 (IL-6) from an antigen-specific (LBRM-33-1A5) and an antigen-nonspecific (EL-4-NOB-1) T-cell line was investigated. rIL-1 beta induced the production of IL-2 and IL-6 from EL-4-NOB-1 cells in a dose-related manner. The LBRM-33-1A5 cells required phytohemagglutinin (PHA) in addition to rIL-1 beta in order to produce IL-2 and IL-6. IL-2 production was found to precede IL-6 production in both cell lines. No IL-2 or IL-6 production was observed by adding r murine tumor necrosis factor-alpha or r murine interferon gamma to the cells. The presence of 1 ng/ml TGF-beta reduced IL-2 and IL-6 production from both T-cell lines by more than 80%. The inhibition of IL-2 and IL-6 production was still evident by a concentration as low as 10 pg/ml of TGF-beta. rIL-1 beta and PHA also stimulated murine thymocytes to produce IL-6 which was inhibited up to 85% in the presence of 1 ng/ml TGF-beta. Taken together these results suggest that TGF-beta may suppress immune responses by inhibiting the endogenous production of IL-2 and IL-6.
研究了重组（r）白细胞介素-1β（rIL-1β）和转化生长因子-β（TGF-β）对一种抗原特异性（LBRM-33-1A5）和一种抗原非特异性（EL-4-NOB-1）T细胞系白细胞介素-2（IL-2）和白细胞介素-6（IL-6）产生的影响。rIL-1β以剂量相关的方式诱导EL-4-NOB-1细胞产生IL-2和IL-6。LBRM-33-1A5细胞除rIL-1β外还需要植物血凝素（PHA）才能产生IL-2和IL-6。在两种细胞系中均发现IL-2的产生先于IL-6的产生。向细胞中添加r小鼠肿瘤坏死因子-α或r小鼠干扰素-γ未观察到IL-2或IL-6的产生。1 ng/ml TGF-β的存在使两种T细胞系的IL-2和IL-6产生减少80%以上。低至10 pg/ml的TGF-β浓度对IL-2和IL-6产生的抑制作用仍然明显。rIL-1β和PHA也刺激小鼠胸腺细胞产生IL-6，在1 ng/ml TGF-β存在的情况下，IL-6的产生被抑制高达85%。综上所述，这些结果表明TGF-β可能通过抑制IL-2和IL-6的内源性产生来抑制免疫反应。
