Viral Emergent Diseases unit, Centre International de Recherches Médicales de Franceville, Franceville, Gabon.
PLoS Pathog. 2012 Sep;8(9):e1002924. doi: 10.1371/journal.ppat.1002924. Epub 2012 Sep 27.
Deep sequencing was used to discover a novel rhabdovirus (Bas-Congo virus, or BASV) associated with a 2009 outbreak of 3 human cases of acute hemorrhagic fever in Mangala village, Democratic Republic of Congo (DRC), Africa. The cases, presenting over a 3-week period, were characterized by abrupt disease onset, high fever, mucosal hemorrhage, and, in two patients, death within 3 days. BASV was detected in an acute serum sample from the lone survivor at a concentration of 1.09 × 10(6) RNA copies/mL, and 98.2% of the genome was subsequently de novo assembled from ≈ 140 million sequence reads. Phylogenetic analysis revealed that BASV is highly divergent and shares less than 34% amino acid identity with any other rhabdovirus. High convalescent neutralizing antibody titers of >1:1000 were detected in the survivor and an asymptomatic nurse directly caring for him, both of whom were health care workers, suggesting the potential for human-to-human transmission of BASV. The natural animal reservoir host or arthropod vector and precise mode of transmission for the virus remain unclear. BASV is an emerging human pathogen associated with acute hemorrhagic fever in Africa.
深度测序用于发现一种新型的弹状病毒（巴桑刚果病毒，或 BASV），该病毒与 2009 年非洲刚果民主共和国（DRC）曼加拉村的 3 例急性出血性发热人类病例爆发有关。这些病例在 3 周的时间内呈现出疾病突然发作、高热、黏膜出血的特征，其中两名患者在 3 天内死亡。BASV 在唯一幸存者的急性血清样本中检测到浓度为 1.09×10(6) RNA 拷贝/mL，随后从约 1.4 亿个序列读长中从头组装了 98.2%的基因组。系统进化分析显示，BASV 高度分化，与任何其他弹状病毒的氨基酸同一性小于 34%。在幸存者和直接照顾他的无症状护士中均检测到高恢复期中和抗体滴度>1:1000，这两人都是医护人员，表明 BASV 可能在人与人之间传播。该病毒的天然动物储存宿主或节肢动物媒介以及确切的传播方式仍不清楚。BASV 是一种与非洲急性出血性发热相关的新兴人类病原体。
