School of Basic Medicine and Biological Sciences, Soochow University, Jiangsu, 215123, People's Republic of China.
Mol Biol Rep. 2012 Dec;39(12):11023-30. doi: 10.1007/s11033-012-2005-1. Epub 2012 Oct 10.
The first complete mitochondrial genome (mitogenome) of Tachinidae Exorista sorbillans (Diptera) is sequenced by PCR-based approach. The circular mitogenome is 14,960 bp long and has the representative mitochondrial gene (mt gene) organization and order of Diptera. All protein-coding sequences are initiated with ATN codon; however, the only exception is Cox I gene, which has a 4-bp ATCG putative start codon. Ten of the thirteen protein-coding genes have a complete termination codon (TAA), but the rest are seated on the H strand with incomplete codons. The mitogenome of E. sorbillans is biased toward A+T content at 78.4 %, and the strand-specific bias is in reflection of the third codon positions of mt genes, and their T/C ratios as strand indictor are higher on the H strand more than those on the L strand pointing at any strain of seven Diptera flies. The length of the A+T-rich region of E. sorbillans is 106 bp, including a tandem triple copies of a13-bp fragment. Compared to Haematobia irritans, E. sorbillans holds distant relationship with Drosophila. Phylogenetic topologies based on the amino acid sequences, supporting that E. sorbillans (Tachinidae) is clustered with strains of Calliphoridae and Oestridae, and superfamily Oestroidea are polyphyletic groups with Muscidae in a clade.
采用 PCR 方法对双翅目寄蝇科外寄蝇属（Exorista）的第一个完整线粒体基因组（mitogenome）进行测序。该环状线粒体基因组长 14960bp，具有双翅目代表性的线粒体基因（mt 基因）组织和排列顺序。所有蛋白质编码序列均以 ATN 密码子起始；然而，唯一的例外是 Cox I 基因，它具有 4bp 的 ATCG 假定起始密码子。13 个蛋白质编码基因中有 10 个具有完整的终止密码子（TAA），但其余的基因位于 H 链上，具有不完整的密码子。外寄蝇的线粒体基因组偏向于 A+T 含量，为 78.4%，链特异性偏向反映了 mt 基因的第三个密码子位置，它们的 T/C 比值作为链指示符，在 H 链上的比值高于 L 链，这表明七种双翅目蝇的任何菌株都存在这种情况。外寄蝇富含 A+T 的区域长度为 106bp，包括串联重复的 13bp 片段的三倍拷贝。与 Haematobia irritans 相比，外寄蝇与果蝇属（Drosophila）的亲缘关系较远。基于氨基酸序列的系统发育拓扑结构表明，外寄蝇（寄蝇科）与丽蝇科（Calliphoridae）和皮蝇科（Oestridae）的菌株聚类，而 Oestroidea 超科是多系群，与 Muscidae 聚类在一起。
