细胞内冰形成的机制。
Mechanisms of intracellular ice formation.
作者信息
Muldrew K, McGann L E
机构信息
Department of Pathology, University of Alberta, Edmonton, Canada.
出版信息
Biophys J. 1990 Mar;57(3):525-32. doi: 10.1016/S0006-3495(90)82568-6.
Abstract
The phenomenon of intracellular freezing in cells was investigated by designing experiments with cultured mouse fibroblasts on a cryomicroscope to critically assess the current hypotheses describing the genesis of intracellular ice: (a) intracellular freezing is a result of critical undercooling; (b) the cytoplasm is nucleated through aqueous pores in the plasma membrane; and (c) intracellular freezing is a result of membrane damage caused by electrical transients at the ice interface. The experimental data did not support any of these theories, but was consistent with the hypothesis that the plasma membrane is damaged at a critical gradient in osmotic pressure across the membrane, and intracellular freezing occurs as a result of this damage. An implication of this hypothesis is that mathematical models can be used to design protocols to avoid damaging gradients in osmotic pressure, allowing new approaches to the preservation of cells, tissues, and organs by rapid cooling.
摘要
通过在低温显微镜上对培养的小鼠成纤维细胞进行实验设计，研究了细胞内结冰现象，以严格评估目前描述细胞内冰形成的假说：(a) 细胞内结冰是临界过冷的结果；(b) 细胞质通过质膜上的水孔成核；(c) 细胞内结冰是冰界面处电瞬变导致膜损伤的结果。实验数据不支持这些理论中的任何一个，但与以下假说一致：质膜在跨膜渗透压的临界梯度下受损，细胞内结冰是这种损伤的结果。该假说的一个推论是，可以使用数学模型来设计方案，以避免渗透压的损伤梯度，从而为通过快速冷却保存细胞、组织和器官提供新方法。
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本文引用的文献
1
Effect of cold acclimation on intracellular ice formation in isolated protoplasts.冷驯化对分离原生质体中细胞内冰晶形成的影响。
Plant Physiol. 1983 Aug;72(4):978-88. doi: 10.1104/pp.72.4.978.
2
Manifestations of injury in yeast cells exposed to subzero temperatures. I. Morphological changes in freeze-substituted and in "frozen-thawed" cells.暴露于零下温度的酵母细胞中的损伤表现。I. 冷冻置换和“冻融”细胞中的形态变化。
J Bacteriol. 1961 Nov;82(5):662-72. doi: 10.1128/jb.82.5.662-672.1961.
3
KINETICS OF WATER LOSS FROM CELLS AT SUBZERO TEMPERATURES AND THE LIKELIHOOD OF INTRACELLULAR FREEZING.零下温度下细胞水分流失的动力学及细胞内结冰的可能性
J Gen Physiol. 1963 Nov;47(2):347-69. doi: 10.1085/jgp.47.2.347.
4
Physical factors implicated in the death of microorganisms at subzero temperatures.与微生物在零下温度下死亡相关的物理因素。
Ann N Y Acad Sci. 1960 Apr 13;85:610-29. doi: 10.1111/j.1749-6632.1960.tb49986.x.
5
Analysis of "solution effects" injury. Equations for calculating phase diagram information for the ternary systems NaCl-dimethylsulfoxide-water and NaCl-glycerol-water.“溶液效应”损伤分析。用于计算三元体系氯化钠 - 二甲基亚砜 - 水和氯化钠 - 甘油 - 水相图信息的方程。
Biophys J. 1980 Nov;32(2):837-50. doi: 10.1016/S0006-3495(80)85019-3.
6
Design and fabrication of a simple, versatile cryomicroscopy stage.一种简单、通用的低温显微镜载物台的设计与制造。
Cryobiology. 1982 Oct;l9(5):529-38. doi: 10.1016/0011-2240(82)90182-1.
7
Interactions of cooling velocity, temperature, and warming velocity on the survival of frozen and thawed yeast.冷却速度、温度和升温速度对冷冻和解冻酵母存活率的相互作用。
Cryobiology. 1968 Jul-Aug;5(1):1-17. doi: 10.1016/s0011-2240(68)80138-5.
8
A two-factor hypothesis of freezing injury. Evidence from Chinese hamster tissue-culture cells.冻伤的双因素假说。来自中国仓鼠组织培养细胞的证据。
Exp Cell Res. 1972;71(2):345-55. doi: 10.1016/0014-4827(72)90303-5.
9
A cryomicroscope for the study of freezing and thawing processes in biological cells.一种用于研究生物细胞冷冻和解冻过程的低温显微镜。
Cryobiology. 1970 Nov-Dec;7(4):191-9. doi: 10.1016/0011-2240(70)90021-0.
10
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Cryobiology. 1973 Jun;10(2):134-40. doi: 10.1016/0011-2240(73)90019-9.
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