Department of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China.
Biomaterials. 2013 Jan;34(1):11-8. doi: 10.1016/j.biomaterials.2012.09.043. Epub 2012 Oct 12.
The phenomenon of light-induced cell detachment is reported. Mouse calvaria-derived, pre-osteoblastic (MC3T3-E1) cells were cultured on a TiO(2) nanodot-coated quartz substrate. After 20 min of UV365 illumination, over 90% of the cells would detach from the surface. Moreover, intact cell sheets could be obtained in the same way. It was found that the as-obtained cells showed good viability, and could be used for further culture processes and other applications. Also, biocompatibility and safety characterizations indicated that the use of TiO(2) nanodots and UV365 illumination was safe for such cell detachment. It is suggested that adsorbed extracellular matrix proteins play key roles in developing cell sheets and ensuring biocompatibility. The present light-induced cell detachment method demonstrates a promising way for rapid cell/cell sheet harvesting.
光诱导细胞脱离现象被报道。将鼠颅骨来源的成骨前体细胞（MC3T3-E1）培养在 TiO2 纳米点涂覆的石英基底上。经过 365nmUV 光照射 20 分钟后，超过 90%的细胞会从表面脱离。此外，同样的方法可以获得完整的细胞片。结果发现，所获得的细胞具有良好的活力，可以用于进一步的培养过程和其他应用。此外，生物相容性和安全性特征表明，使用 TiO2 纳米点和 365nmUV 光照射对于这种细胞脱离是安全的。研究表明，吸附的细胞外基质蛋白在细胞片的形成和保证生物相容性方面起着关键作用。本研究提出的光诱导细胞脱离方法为快速收获细胞/细胞片提供了一种有前途的途径。
