Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Agronomical Sciences and Veterinary Medicine, Bucharest, Romania.
Foodborne Pathog Dis. 2012 Nov;9(11):980-5. doi: 10.1089/fpd.2012.1237. Epub 2012 Oct 17.
An epidemiological survey of cystic echinococcosis (CE) in cattle and sheep was conducted from October 2009 to October 2011 in endemic areas of north-eastern and southern Romania. A total of 8569 animals (3043 cattle and 5526 sheep) were examined, and hydatid cysts were found in 984 (32.34%) cattle and 2756 (49.87%) sheep, respectively. The average number of cysts per animal was 11.3 (range, 1-47) in cattle and 4.2 (range, 1-16) in sheep. Of the CE-positive animals, 177 (17.9%) of the cattle and 358 (12.98%) of the sheep had hydatid cysts only in the lungs, 62 (6.3%) and 803 (29.13%) had only in the liver, and 723 (73.47%) and 1572 (57.04%), respectively, had hydatid cysts both in the liver and in the lungs. Very few animals had cysts in spleen (19 cattle and 23 sheep) and kidney (three cattle). From a sample of 422 cysts undergoing closer examination (258 from cattle, 164 from sheep), the highest fertility rate (38.41%) was found in sheep cysts, while from the cattle only four cysts (1.55%) were found to be fertile. Species identification of 13 animal isolates (nine from sheep, four from cattle) and one human isolate were done by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the mitochondrial nad1 gene and confirmed by partial sequencing of the cox1 gene. All showed the same RFLP band pattern (Echinococcus granulosus sensu stricto) and were identified as the "sheep strain" G1 by sequencing. The study emphasizes the hyperendemic presence of E. granulosus in Romania and outlines the necessity for the urgent development of sustainable surveillance and control strategies both in animals and humans.
2009 年 10 月至 2011 年 10 月，在罗马尼亚东北部和南部的流行地区对牛和羊的囊型包虫病（CE）进行了流行病学调查。共检查了 8569 只动物（3043 头牛和 5526 只羊），分别在 984 只（32.34%）牛和 2756 只（49.87%）羊中发现了包虫囊肿。每只动物的平均包虫囊肿数为 11.3 个（范围 1-47）牛和 4.2 个（范围 1-16）羊。在 CE 阳性动物中，177 只（17.9%）牛和 358 只（12.98%）羊的包虫囊肿仅在肺部，62 只（6.3%）和 803 只（29.13%）仅在肝脏，723 只（73.47%）和 1572 只（57.04%）分别在肝脏和肺部有包虫囊肿。很少有动物的脾脏（19 头牛和 23 只羊）和肾脏（3 头牛）有囊肿。从进一步检查的 422 个囊肿样本（258 个来自牛，164 个来自羊）中发现，绵羊囊肿的最高生育率（38.41%），而仅从牛中发现了 4 个囊肿（1.55%）是可育的。通过线粒体 nad1 基因聚合酶链反应-限制性片段长度多态性（PCR-RFLP）对 13 个动物分离株（9 个来自绵羊，4 个来自牛）和 1 个人类分离株进行了种属鉴定，并通过 cox1 基因的部分测序得到证实。所有结果均显示出相同的 RFLP 带型（细粒棘球蚴），并通过测序鉴定为“绵羊株”G1。该研究强调了罗马尼亚的包虫病高度流行，并概述了在动物和人类中紧急制定可持续监测和控制策略的必要性。
