Kotani H, Butler G H, Tallarida D, Cody C, McGarrity G J
Coriell Institute for Medical Research, Camden, New Jersey 08103.
In Vitro Cell Dev Biol. 1990 Jan;26(1):91-6. doi: 10.1007/BF02624161.
The failure of many cell culture isolates of Mycoplasma hyorhinis to grow on microbiological media has stressed the need for alternate assays to detect these organisms. The use of freshly prepared yeast extract in mycoplasmal media together with incubation in 5% CO2/air successfully detected M. hyorhinis in 12 of 12 infected cultures. These were not detected by the use of conventional mycoplasmal media using aerobic or anaerobic incubation. This assay may also be helpful in detection of other mycoplasmal species commonly isolated from cell cultures.
许多猪鼻支原体细胞培养分离株无法在微生物培养基上生长，这凸显了采用替代检测方法来检测这些微生物的必要性。在支原体培养基中使用新鲜制备的酵母提取物并在5%二氧化碳/空气环境中孵育，成功在12份受感染培养物中的12份中检测到了猪鼻支原体。而使用传统支原体培养基进行需氧或厌氧孵育则未检测到这些菌株。该检测方法可能也有助于检测其他常见于细胞培养物中的支原体物种。
