比较研究以确定6-甲基嘌呤脱氧核糖苷检测细胞培养支原体的效率。

Comparative studies to determine the efficiency of 6 methylpurine deoxyriboside to detect cell culture mycoplasmas.

作者信息

McGarrity G J, Kotani H, Carson D

出版信息

In Vitro Cell Dev Biol. 1986 Jun;22(6):301-4. doi: 10.1007/BF02623401.

Abstract

Studies were performed to compare three methods to detect mycoplasmal infection of cell cultures. The methods included microbiological assay by inoculation into broth and onto agar with anaerobic incubation, fluorescent DNA staining by Hoechst 33258, and mycoplasmal mediated cytotoxicity by 6 methylpurine deoxyriboside (6MPDR). Fluorescent DNA staining and 6MPDR assays were performed in an indicator cell culture system. A total of 2589 cell cultures were assayed. Mycoplasmas were detected in 174, an incidence of 6.7%. Species isolated were: Acholeplasma laidlawii, Mycoplasma orale, M. arginini, M. hyorhinis, M. fermentans, M. pirum, and M. pneumoniae. In separate studies, 6MPDR also detected infection with Spiroplasma mirum when this organism was deliberately inoculated into cell cultures. The efficiencies of microbiological testing, fluorescent DNA assays, and 6MPDR were 43.1, 98.8, and 97.1%, respectively.

摘要

开展了多项研究以比较三种检测细胞培养物支原体感染的方法。这些方法包括接种到肉汤和琼脂上进行厌氧培养的微生物学检测、用Hoechst 33258进行的荧光DNA染色，以及用6-甲基嘌呤脱氧核糖苷（6MPDR）进行的支原体介导的细胞毒性检测。荧光DNA染色和6MPDR检测在指示细胞培养系统中进行。总共检测了2589份细胞培养物。在174份中检测到支原体，发生率为6.7%。分离出的菌种有：莱氏无胆甾原体、口腔支原体、精氨酸支原体、猪鼻支原体、发酵支原体、梨支原体和肺炎支原体。在单独的研究中，当将微小螺旋体故意接种到细胞培养物中时，6MPDR也检测到了该菌的感染。微生物检测、荧光DNA检测和6MPDR的效率分别为43.1%、98.8%和97.1%。