Max-Planck Institute for Medical Research, Heidelberg, Germany.
Nat Methods. 2012 Dec;9(12):1198-201. doi: 10.1038/nmeth.2213. Epub 2012 Oct 21.
The development of methods for imaging large contiguous volumes with the electron microscope could allow the complete mapping of a whole mouse brain at the single-axon level. We developed a method based on prolonged immersion that enables staining and embedding of the entire mouse brain with uniform myelin staining and a moderate preservation of the tissue's ultrastructure. We tested the ability to follow myelinated axons using serial block-face electron microscopy.
发展电子显微镜对大的连续体积进行成像的方法可以使我们能够在单个轴突水平上对整个老鼠大脑进行完整的绘图。我们开发了一种基于长时间浸泡的方法，该方法可以对整个老鼠大脑进行均匀的髓鞘染色和组织超微结构的适度保存。我们使用连续块面电子显微镜来测试跟踪有髓轴突的能力。
