Department of Ecology, Institute of Hydrobiology, Jinan University, Guangzhou 510632, China.
Chemosphere. 2013 Jan;90(3):1281-8. doi: 10.1016/j.chemosphere.2012.09.087. Epub 2012 Oct 23.
The toxic effects of triclosan (TCS) on the swordtail fish (Xiphophorus helleri) were assessed based on various biomarkers including enzymatic activities of ethoxyresorufin O-deethylase (EROD), erythromycin N-demethylase (ERND) and glutathione-s-transferase (GST) and mRNA expression levels of CYP1A, CYP3A, glutathione S-transferase (GST) and P-glycoprotein (P-gp). The acute toxicity test showed the LC(50) value of 1.47 mg L(-1) for TCS. The mRNA expressions of CYP1A, CYP3A, GST and P-gp showed dose-effect relationships in female swordtail fish when exposed to TCS, These mRNA expression levels were found more sensitive to TCS exposure than the enzymatic activities of EROD, ERND and GST do. In addition, the male fish displayed higher gene expression levels and more dramatic changes in enzyme activities than the females did. Our data further demonstrated that TCS was a typical inducer to Phase I and Phase II metabolism enzymes and genes, suggesting it is a potential ecotoxicological risk to aquatic ecosystems.
基于包括对乙氧基resorufin O-去乙基酶（EROD）、红霉素 N-去甲基酶（ERND）和谷胱甘肽-S-转移酶（GST）的酶活性以及细胞色素 P4501A（CYP1A）、细胞色素 P4503A（CYP3A）、谷胱甘肽 S-转移酶（GST）和 P-糖蛋白（P-gp）的 mRNA 表达水平等各种生物标志物，评估了三氯生（TCS）对剑尾鱼（Xiphophorus helleri）的毒性作用。急性毒性试验表明，TCS 的 LC（50）值为 1.47mg/L。当剑尾鱼暴露于 TCS 时，CYP1A、CYP3A、GST 和 P-gp 的 mRNA 表达呈现出剂量-效应关系，这些 mRNA 表达水平比 EROD、ERND 和 GST 的酶活性对 TCS 暴露更为敏感。此外，雄性鱼表现出比雌性鱼更高的基因表达水平和更剧烈的酶活性变化。我们的数据进一步表明，TCS 是一种典型的 I 相和 II 相代谢酶和基因诱导物，表明它对水生生态系统是一种潜在的生态毒理学风险。
