Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, China.
Int Immunopharmacol. 2012 Dec;14(4):758-63. doi: 10.1016/j.intimp.2012.10.006. Epub 2012 Oct 22.
This study was conducted to evaluate the in vitro immunomodulatory effects of supercritical CO(2)Ligustrum lucidum extract (LLE) on the immune cells of piglets. The results showed that the LLE enhanced the proliferative activity of piglet blood lymphocytes and up-regulated the CD4(+) CD8(+) and CD4(+) CD8(-) cell populations. The LLE also regulated the expression of Th1- and Th2-related cytokines; elevated the levels of IL-2, IFN-γ and TNF-α, which were produced by Th1 lymphocytes; and decreased the levels of IL-4 and IL-10, which were produced by Th2 lymphocytes. Furthermore, the LLE stimulated the NO secretion of lymphocytes. These results indicated that LLE might have potential immunomodulatory effects on the immune system of piglets and provided scientific and experimental foundations for the development of a new kind of LLE immune adjuvant in the pig production.
本研究旨在评估超临界 CO(2)女贞子提取物（LLE）对仔猪免疫细胞的体外免疫调节作用。结果表明，LLE 增强了仔猪血液淋巴细胞的增殖活性，并上调了 CD4(+) CD8(+)和 CD4(+) CD8(-)细胞群。LLE 还调节了 Th1 和 Th2 相关细胞因子的表达；提高了 Th1 淋巴细胞产生的 IL-2、IFN-γ 和 TNF-α 的水平；降低了 Th2 淋巴细胞产生的 IL-4 和 IL-10 的水平。此外，LLE 刺激了淋巴细胞的 NO 分泌。这些结果表明，LLE 可能对仔猪的免疫系统具有潜在的免疫调节作用，并为猪生产中新型 LLE 免疫佐剂的开发提供了科学和实验基础。
