Department of Biotechnology and Food Engineering, School of Biotechnology and Food, Centro de Biotecnología-FEMSA, Tecnológico de Monterrey-Campus Monterrey, E. Garza Sada 2501 Sur, CP 64849 Monterrey, NL, Mexico.
J Agric Food Chem. 2012 Nov 14;60(45):11378-86. doi: 10.1021/jf303252v. Epub 2012 Nov 5.
The use of plants to produce chemical compounds with pharmaceutical and nutraceutical applications has intensified in recent years. In this regard, genetic engineering is the most commonly used tool to generate crop lines with enhanced concentrations of desirable chemicals. However, growing genetically modified plants is still limited because they are perceived as potential biological hazards that can create an ecological imbalance. The application of postharvest abiotic stresses on plants induces the accumulation of secondary metabolites and thus can be used as an alternative to genetic modification. The present project evaluated the feasibility of producing shikimic acid (SA) and phenolic compounds (PC) in wounded carrots ( Daucus carota ) treated with glyphosate. The spray application of a concentrated glyphosate solution on wounded carrot tissue increased the concentrations of SA and chlorogenic acid by ∼1735 and ∼5700%, respectively. The results presented herein demonstrate the potential of stressed carrot tissue as a biofactory of SA and PC.
近年来，利用植物生产具有药用和营养应用的化合物的方法日益增多。在这方面，遗传工程是最常用的工具，可用于生成具有理想化学物质浓度的作物品系。然而，由于人们认为转基因植物可能是潜在的生物危害，会造成生态失衡，因此种植它们仍然受到限制。对植物施加采后非生物胁迫会诱导次生代谢物的积累，因此可以作为遗传修饰的替代方法。本项目评估了利用草甘膦处理受伤胡萝卜（ Daucus carota ）来生产莽草酸（SA）和酚类化合物（PC）的可行性。将浓缩的草甘膦溶液喷洒在受伤的胡萝卜组织上，分别使 SA 和绿原酸的浓度增加了约 1735%和 5700%。本文的研究结果表明，应激胡萝卜组织作为 SA 和 PC 的生物工厂具有一定的潜力。
