纳米颗粒跟踪分析技术用于总病毒粒子测定的评估。
Evaluation of nanoparticle tracking analysis for total virus particle determination.
机构信息
BIA Separations, d,o,o, Mirce 21, SI-5270, Ajdovščina, Slovenia.
出版信息
Virol J. 2012 Nov 12;9:265. doi: 10.1186/1743-422X-9-265.
The NanoSight LM10 with Nanoparticle tracking analysis (NTA) software was evaluated for the quantification of latex particles, adenovirus 5, and influenza virus. The inter-day variability was determined by measuring the same sample over several consecutive days and the method's accuracy was demonstrated by using known concentrations of the subject particles. NTA analysis was also used to quantify chromatographic fractions of adenovirus and influenza virus after purification on a CIM monolithic column. NTA results were compared and evaluated against hemagglutination (HA) and end point dilution assay, determining total and infection virus particle number, respectively. The results demonstrated that nanoparticle tracking analysis is a method for fast estimation of virus concentration in different samples. In addition, it can provide a better insight into the sample status, regarding the level of virus aggregation.
采用纳米粒度及分子运动分析仪（NanoSight LM10）及其纳米颗粒跟踪分析（NTA）软件，对乳胶颗粒、腺病毒 5 型（adenovirus 5）和流感病毒进行定量分析。通过连续几天测量同一样品，评估日间差异；通过使用已知浓度的目标颗粒，验证方法的准确性。在 CIM 整体柱上进行纯化后，还使用 NTA 分析对腺病毒和流感病毒的色谱馏分进行定量。将 NTA 结果与血凝（HA）和终点稀释测定进行比较和评估，分别确定总病毒颗粒数和感染性病毒颗粒数。结果表明，纳米颗粒跟踪分析是一种快速估计不同样品中病毒浓度的方法。此外，它还可以更深入地了解样品状态，包括病毒聚集水平。
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