Department of Ophthalmology, Jules Stein Eye Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Invest Ophthalmol Vis Sci. 2013 Jan 2;54(1):9-18. doi: 10.1167/iovs.12-10241.
We describe infrared regional pupillometry as an objective comparative assessment of midperipheral to central retinal sensitivity and to correlate with midperipheral retinal ischemia in diabetic subjects.
We tested 12 normal and 17 diabetic subjects using bilateral infrared pupillometry. The diabetic cohort included seven subjects without, five with mild, three with moderate, and two with severe non-proliferative diabetic retinopathy (NPDR). Central and annular stimuli of varying intensity were presented to one eye, and pupillary amplitude and constriction velocity were measured from both eyes. Light stimulus of increasing intensity was presented as 20 consecutive trials (stimulus duration of 300 ms with 3000 ms intervals). The ratio of central to peripheral responses (Q values) was calculated for each stimulus configuration. Average responses with respect to the stimulus strength were regressed with Gompertz sigmoid function.
Control and moderate/severe NPDR cases comparison showed statistically significant differences in amplitude (Q(A)) and constriction velocity (Q(CV)) (Wilcoxon rank sum test P = 0.002, respectively). Age difference for these groups was not statistically significant (Wilcoxon rank sum test P = 0.15). The comparison of control and diabetic subjects without NPDR/mild NPDR was statistically significant for Q(A) and Q(CV) (Wilcoxon rank sum test P = 0.0002 and P = 0.001, respectively). Q(A) and Q(CV) differences were statistically significant between moderate/severe NPDR cases and subjects without or mild NPDR cases (Wilcoxon rank sum test P = 0.013).
Q(A) and Q(CV) values correlated highly with the severity of diabetic retinopathy, but not with the duration of diabetes.
我们描述了红外区域性瞳孔描记术，作为一种客观的对比评估方法，用于评估中周边至中心视网膜的敏感性，并与糖尿病患者的中周边视网膜缺血相关联。
我们使用双侧红外瞳孔描记术测试了 12 名正常人和 17 名糖尿病患者。糖尿病组包括 7 名无、5 名轻度、3 名中度和 2 名重度非增生性糖尿病视网膜病变（NPDR）患者。向一只眼呈现不同强度的中央和环形刺激，同时测量双眼的瞳孔幅度和收缩速度。强度递增的光刺激作为 20 次连续试验呈现（刺激持续时间为 300ms，间隔 3000ms）。为每个刺激配置计算中央与周边反应的比率（Q 值）。根据刺激强度对平均反应进行回归，采用 Gompertz 型曲线函数。
控制组和中度/重度 NPDR 病例的比较显示，在幅度（Q(A))和收缩速度（Q(CV))方面存在统计学显著差异（Wilcoxon 秩和检验 P = 0.002，分别）。这些组的年龄差异无统计学意义（Wilcoxon 秩和检验 P = 0.15）。在无 NPDR/轻度 NPDR 的糖尿病患者中，控制组和对照组之间的 Q(A)和 Q(CV)比较具有统计学意义（Wilcoxon 秩和检验 P = 0.0002 和 P = 0.001，分别）。中度/重度 NPDR 病例与无 NPDR/轻度 NPDR 病例之间的 Q(A)和 Q(CV)差异具有统计学意义（Wilcoxon 秩和检验 P = 0.013）。
Q(A)和 Q(CV)值与糖尿病视网膜病变的严重程度高度相关，但与糖尿病的持续时间无关。
