Research Center for Analytical Sciences, Northeastern University, Box 332, Shenyang 110004, China.
Nanoscale. 2013 Jan 7;5(1):246-52. doi: 10.1039/c2nr32457a. Epub 2012 Nov 16.
A hybrid bactericidal material, gold nanorod-covered kanamycin-loaded hollow SiO(2) (HSKAu(rod)) nanocapsules, is constructed. The hybrid material combines the features of a chemical drug with photothermal physical sterilization which decreases the dosage of broad-spectrum antibiotic and the physical damage of biological systems. Hollow SiO(2) nanocapsules are used as carriers for drug delivery. The nanocapsules load a model drug, kanamycin, and are covered with gold nanorods to avoid drug leakage and realize photothermal treatment. The sterilizing effect on the bacterial strain is investigated by incubating E. coli BL21 with the hybrid nanocapsules and irradiating under near-infrared light (NIR) for 20 min. A bactericidal effect, i.e., a sterilizing rate of 53.47%, is achieved for the HSKAu(rod) nanocapsules under NIR irradiation, with respect to a net sum sterilizing rate of 34.49% for the individual components of the HSKAu(rod) nanocapsules, e.g., carrier nanocapsules, chemical sterilization of kanamycin and physical sterilization due to the gold nanorods under NIR irradiation. It is demonstrated that the combination of chemical drug and physical sterilization results in an obvious synergistic effect and makes the sterilization more effective. This novel hybrid has great potential as an adjuvant therapeutic alternative material for sterilization or even for the control of disease.
构建了一种混合杀菌材料，即金纳米棒覆盖的载有卡那霉素的中空 SiO₂（HSKAu（rod））纳米胶囊。该混合材料结合了化学药物和光热物理杀菌的特点，减少了广谱抗生素的用量和对生物系统的物理损伤。中空 SiO₂纳米胶囊用作药物递送载体。纳米胶囊装载模型药物卡那霉素，并覆盖金纳米棒，以避免药物泄漏并实现光热治疗。通过将大肠杆菌 BL21 与混合纳米胶囊孵育并用近红外光（NIR）照射 20 分钟来研究其对细菌菌株的杀菌效果。在 NIR 照射下，HSKAu（rod）纳米胶囊的杀菌效果达到 53.47%，相对于 HSKAu（rod）纳米胶囊的各个组成部分的总净杀菌率 34.49%，例如载体纳米胶囊、卡那霉素的化学杀菌和金纳米棒在 NIR 照射下的物理杀菌。结果表明，化学药物和物理杀菌的结合产生了明显的协同作用，使杀菌更有效。这种新型混合体具有作为辅助治疗性替代材料用于杀菌甚至控制疾病的巨大潜力。
