天然抗菌肽的新视角：作为抗炎药物在小鼠模型中的应用。
New perspectives for natural antimicrobial peptides: application as antinflammatory drugs in a murine model.
机构信息
Faculty of Biotechnology, University of Naples Federico II, Naples 80134, Italy.
出版信息
BMC Immunol. 2012 Nov 17;13:61. doi: 10.1186/1471-2172-13-61.
BACKGROUND
Antimicrobial peptides (AMPs) are an ancient group of defense molecules. AMPs are widely distributed in nature (being present in mammals, birds, amphibians, insects, plants, and microorganisms). They display bactericidal as well as immunomodulatory properties. The aim of this study was to investigate the antimicrobial and anti-inflammatory activities of a combination of two AMPs (temporin B and the royal jellein I) against Staphylococcus epidermidis.
RESULTS
The temporin B (TB-KK) and the royal jelleins I, II, III chemically modified at the C terminal (RJI-C, RJII-C, RJIII-C), were tested for their activity against 10 different Staphylococcus epidermidis strains, alone and in combination. Of the three royal jelleins, RJI-C showed the highest activity. Moreover, the combination of RJI-C and TB-KK (MIX) displayed synergistic activity. In vitro, the MIX displayed low hemolytic activity, no NO2- production and the ability to curb the synthesis of the pro-inflammatory cytokines TNF-α and IFN-γ to the same extent as acetylsalicylic acid. In vivo, the MIX sterilized mice infected with Staphylococcus epidermidis in eleven days and inhibited the expression of genes encoding the prostaglandin-endoperoxide synthase 2 (COX-2) and CD64, two important parameters of inflammation.
CONCLUSION
The study shows that the MIX - a combination of two naturally occurring peptides - displays both antimicrobial and anti-inflammatory activities.
背景
抗菌肽（AMPs）是一组古老的防御分子。AMP 广泛存在于自然界中（存在于哺乳动物、鸟类、两栖动物、昆虫、植物和微生物中）。它们具有杀菌和免疫调节特性。本研究旨在研究两种 AMP（temporin B 和皇家 jellyin I）组合对表皮葡萄球菌的抗菌和抗炎活性。
结果
对化学修饰 C 末端的 temporin B（TB-KK）和三种皇家 jellyin（RJI-C、RJII-C、RJIII-C）进行了测试，以评估它们对 10 种不同表皮葡萄球菌株的活性，单独使用和组合使用。在三种皇家 jellyin 中，RJI-C 表现出最高的活性。此外，RJI-C 和 TB-KK 的组合（MIX）显示出协同活性。体外，MIX 显示出低的溶血活性、不产生 NO2-，并且能够以与乙酰水杨酸相同的程度抑制促炎细胞因子 TNF-α和 IFN-γ的合成。体内，MIX 在 11 天内使表皮葡萄球菌感染的小鼠无菌，并抑制了前列腺素内过氧化物合酶 2（COX-2）和 CD64 的基因表达，这是炎症的两个重要参数。
结论
研究表明，MIX-两种天然存在的肽的组合-具有抗菌和抗炎活性。
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