零售市场上芬兰禽肉产品中的肠外致病性大肠杆菌。
Extraintestinal pathogenic Escherichia coli in poultry meat products on the Finnish retail market.
机构信息
Ruralia Institute, University of Helsinki, Kampusranta 9C, FI-60320, Seinäjoki, Finland.
出版信息
Acta Vet Scand. 2012 Nov 16;54(1):64. doi: 10.1186/1751-0147-54-64.
BACKGROUND
Extraintestinal pathogenic Escherichia coli bacteria (ExPEC) exist as commensals in the human intestines and can infect extraintestinal sites and cause septicemia. The transfer of ExPEC from poultry to humans and the role of poultry meat as a source of ExPEC in human disease have been discussed previously. The aim of the present study was to provide insight into the properties of ExPEC in poultry meat products on the Finnish retail market with special attention to their prevalence, virulence and phylogenetic profiles. Furthermore, the isolates were screened for possible ESBL producers and their resistance to nalidixic acid and ciprofloxacin was tested.
METHODS
The presence of ExPEC in 219 marinated and non-marinated raw poultry meat products from retail shops has been analyzed. One E. coli strain per product was analyzed further for phylogenetic groups and possession of ten virulence genes associated with ExPEC bacteria (kpsMT K1, ibeA, astA, iss, irp2, papC, iucD, tsh, vat and cva/cv) using PCR methods. The E. coli strains were also screened phenotypically for the production of extended-spectrum β-lactamase (ESBL) and the susceptibility of 48 potential ExPEC isolates for nalidixic acid and ciprofloxacin was tested.
RESULTS
E. coli was isolated from 207 (94.5%) of 219 poultry meat products. The most common phylogenetic groups were D (50.7%), A (37.7%), and B2 (7.7%). Based on virulence factor gene PCR, 23.2% of the strains were classified as ExPEC. Two ExPEC strains (1%) belonged to [O1] B2 svg+ (specific for virulent subgroup) group, which has been implicated in multiple forms of ExPEC disease. None of the ExPEC strains was resistant to ciprofloxacin or cephalosporins. One isolate (2.1%) showed resistance to nalidixic acid.
CONCLUSIONS
Potential ExPEC bacteria were found in 22% of marinated and non-marinated poultry meat products on the Finnish retail market and 0.9% were contaminated with E. coli [O1] B2 svg+ group. Marinades did not have an effect on the survival of ExPEC as strains from marinated and non-marinated meat products were equally often classified as ExPEC. Poultry meat products on the Finnish retail market may have zoonotic potential.
背景
肠外致病性大肠杆菌（ExPEC）作为人类肠道中的共生菌存在，可感染肠道外部位并引起败血症。此前已经讨论了 ExPEC 从家禽转移到人类以及禽肉作为人类疾病中 ExPEC 来源的作用。本研究的目的是深入了解芬兰零售市场上禽肉产品中 ExPEC 的特性，特别关注其流行率、毒力和系统发育谱。此外，还筛选了分离株是否为可能的 ESBL 生产者，并测试了它们对奈啶酸和环丙沙星的耐药性。
方法
分析了来自零售商店的 219 种腌制和非腌制生禽肉产品中 ExPEC 的存在情况。对每种产品中的一株大肠杆菌菌株进行了进一步分析，以确定其与 ExPEC 细菌相关的十个毒力基因（kpsMT K1、ibeA、astA、iss、irp2、papC、iucD、tsh、vat 和 cva/cv）的存在情况，并使用 PCR 方法确定其系统发育群。还通过表型筛选法筛选了大肠杆菌菌株对扩展谱β-内酰胺酶（ESBL）的产生情况，并测试了 48 株潜在 ExPEC 分离株对奈啶酸和环丙沙星的敏感性。
结果
从 219 种禽肉产品中分离出 207 株（94.5%）大肠杆菌。最常见的系统发育群是 D（50.7%）、A（37.7%）和 B2（7.7%）。基于毒力因子基因 PCR，23.2%的菌株被归类为 ExPEC。两种 ExPEC 菌株（1%）属于[O1]B2 svg+（特定于毒力亚群）组，该组与多种形式的 ExPEC 疾病有关。没有 ExPEC 菌株对环丙沙星或头孢菌素耐药。一株分离株（2.1%）对萘啶酸耐药。
结论
在芬兰零售市场的腌制和非腌制禽肉产品中，有 22%发现了潜在的 ExPEC 细菌，有 0.9%受到了大肠杆菌[O1]B2 svg+群的污染。腌料并没有影响 ExPEC 的存活，因为来自腌制和非腌制肉类产品的菌株同样经常被归类为 ExPEC。芬兰零售市场上的禽肉产品可能具有动物源性。
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