Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-756, Republic of Korea.
Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Jun;63(Pt 6):2314-2319. doi: 10.1099/ijs.0.044099-0. Epub 2012 Nov 16.
Strain LHWP2(T), a novel, aerobic, budding, motile and ovoid bacterium belonging to the phylum Planctomycetes, was isolated from a dead ark clam (Scapharca broughtonii) from the south coast of Korea. Strain LHWP2(T) grew optimally at 30 °C, in the presence of 4% (w/v) NaCl, and at pH 7. The predominant cellular fatty acids were C16:0, C18:1ω7c and/or C18:1ω6c (summed feature 8) and C18:1ω9c. The major isoprenoid quinone was menaquinone-6 (MK-6). The dominant polar lipid was identified as phosphatidylglycerol. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel strain was most closely related to Blastopirellula marina DSM 3645(T), with a 16S rRNA gene sequence similarity of 94.1%. The genomic DNA G+C content of strain LHWP2(T) was 49.5 mol%. Strain LHWP2(T) was distinguished from B. marina DSM 3645(T) based on its optimum salinity, acid production from substrates, assimilation of substrates and DNA G+C content. Overall, these phenotypic, genotypic and phylogenetic data suggest that strain LHWP2(T) should be classified as a novel species belonging to the genus Blastopirellula, for which the name Blastopirellula cremea sp. nov. is proposed. The type strain is LHWP2(T) (=KACC 15559(T)=JCM 17758(T)).
菌株 LHWP2(T)，是一种新型、需氧、出芽、能动、卵形的细菌，属于浮霉状菌门。它是从韩国南部海岸死亡的褶牡蛎（Scapharca broughtonii）中分离出来的。菌株 LHWP2(T) 在 30°C、4%（w/v）NaCl 和 pH7 的条件下生长最佳。主要细胞脂肪酸为 C16:0、C18:1ω7c 和/或 C18:1ω6c（总和特征 8）和 C18:1ω9c。主要类异戊二烯醌为甲萘醌-6（MK-6）。主要极性脂为磷脂酰甘油。基于 16S rRNA 基因序列的系统发育分析表明，该新型菌株与 Blastopirellula marina DSM 3645(T)最为密切相关，16S rRNA 基因序列相似度为 94.1%。菌株 LHWP2(T) 的基因组 DNA G+C 含量为 49.5mol%。根据最适盐度、底物产酸、底物同化和 DNA G+C 含量，菌株 LHWP2(T)与 Blastopirellula marina DSM 3645(T)区分开来。总的来说，这些表型、基因型和系统发育数据表明，菌株 LHWP2(T)应被归类为 Blastopirellula 属的一个新型物种，建议将其命名为 Blastopirellula cremea sp. nov.。模式菌株为 LHWP2(T)=KACC 15559(T)=JCM 17758(T)。
