Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 130-701, Republic of Korea.
J Microbiol. 2013 Jun;51(3):301-4. doi: 10.1007/s12275-013-3210-x. Epub 2013 Jun 28.
A novel Gram-negative, motile, and ovoid-shaped strain, LHWP3(T), which belonged to the family Planctomycetaceae in the phylum Planctomycetes, was isolated from a dead ark clam Scapharca broughtonii collected during a mass mortality event on the south coast of Korea. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the isolate was most closely related to the type strain of Rhodopirellula baltica, with a shared 16S rRNA gene sequence similarity of 94.8%. The isolate grew optimally at 30°C in 4-6% (w/v) NaCl, and at pH 7. The major isoprenoid quinone was menaquinone-6 (MK-6). The dominant polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, and unidentified polar lipids. The predominant cellular fatty acids were C16:0, C18:1 ω9c, and C18:0. The genomic DNA G+C content of strain LHWP3(T) was 53.0 mol%. Based on polyphasic taxonomic analyses, strain LHWP3(T) should be classified as a novel species in the genus Rhodopirellula in the family Planctomycetaceae, for which the name Rhodopirellula rosea sp. nov. is proposed. The type strain is LHWP3(T) (=KACC 15560(T) =JCM 17759(T)).
一株新的革兰氏阴性、能动、卵形的菌株 LHWP3(T)，属于浮霉状菌科（Planctomycetaceae）的浮霉状菌门（Planctomycetes），是从韩国南部海岸在大规模死亡事件中采集的死贝壳 Scapharca broughtonii 中分离出来的。基于 16S rRNA 基因序列的系统发育分析表明，该分离株与 Rhodopirellula baltica 的模式株最为密切相关，具有 94.8%的共享 16S rRNA 基因序列相似性。该分离株在 30°C、4-6%（w/v）NaCl 和 pH 7 下生长最佳。主要的类异戊二烯醌是甲萘醌-6（MK-6）。主要的极性脂是磷脂酰胆碱、磷脂酰甘油、磷脂酰乙醇胺和未鉴定的极性脂。主要的细胞脂肪酸是 C16:0、C18:1 ω9c 和 C18:0。菌株 LHWP3(T) 的基因组 DNA G+C 含量为 53.0 mol%。基于多相分类学分析，菌株 LHWP3(T) 应归类为浮霉状菌科浮霉状菌属中的一个新种，命名为 Rhodopirellula rosea sp. nov.。模式株为 LHWP3(T)（=KACC 15560(T) =JCM 17759(T)）。
