Centre for Complementary Medicine Research, University of Western Sydney, Locked Bag 1797, Penrith South DC, NSW 1797, Australia.
Chin Med. 2012 Nov 24;7(1):26. doi: 10.1186/1749-8546-7-26.
This study aims to determine the relationship between the antioxidant and anti-inflammatory activities of the thirteen herbs and two fungi extracts, and their total phenolic and flavonoid contents.
Antioxidant activities were evaluated by four assays: an antioxidant activity assay using Saccharomyces cerevisiae, a DPPH ((2, 2-diphenyl-1-picrylhydrazyl) assay to assess free radical scavenging, an assay assessing ferrous ions or iron (II) chelating ability, and a ferric reducing antioxidant power (FRAP) assay. Total phenolic and flavonoid contents were determined using the Folin-Ciocalteu and aluminium chloride methods, respectively. Anti-inflammatory activities were determined by measuring the inhibition of nitric oxide and TNF-α production in lipopolysaccharide- and interferon-γ-activated J774A.1 macrophages. Their cytotoxicities against macrophages were determined by MTT assay.
A positive linear correlation between antioxidant activities and the total phenolic and flavonoid content of the plant extracts was found. The plant extracts with high phenolic and flavonoid content also exhibited significant anti-inflammatory activity with good cell viability.
The selected herbs could be a rich source of antioxidants and free radical scavenging compounds. The levels of phenolic and flavonoid compounds were correlated with the antioxidant and anti-inflammatory activities of the extracts from the herbs.
本研究旨在确定十三味草药和两种真菌提取物的抗氧化和抗炎活性与其总酚类和类黄酮含量之间的关系。
采用四种方法评估抗氧化活性：使用酿酒酵母的抗氧化活性测定法、评估自由基清除能力的 DPPH（2,2-二苯基-1-苦基肼基）测定法、评估亚铁离子或铁(II)螯合能力的测定法以及铁还原抗氧化能力（FRAP）测定法。总酚类和类黄酮含量分别采用福林-考尔法和三氯化铝法测定。采用 LPS 和 IFN-γ 激活的 J774A.1 巨噬细胞测定抗炎活性，以抑制一氧化氮和 TNF-α的产生。通过 MTT 测定法测定对巨噬细胞的细胞毒性。
发现抗氧化活性与植物提取物中总酚类和类黄酮含量之间存在正线性相关。酚类和类黄酮含量高的植物提取物也表现出显著的抗炎活性和良好的细胞活力。
所选草药可能是抗氧化剂和自由基清除化合物的丰富来源。酚类和类黄酮化合物的水平与草药提取物的抗氧化和抗炎活性相关。
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