Department of Ophthalmology, Shimane University Faculty of Medicine, Izumo, Shimane, Japan.
PLoS One. 2012;7(11):e49680. doi: 10.1371/journal.pone.0049680. Epub 2012 Nov 26.
The involvement of local and systemic oxidative stress in intraocular pressure (IOP) elevation and optic nerve damage has been hypothesized in the pathogenesis of glaucoma. To test this, we measured the systemic levels of prooxidants and antioxidants by analyzing the blood biochemistry in patients with glaucoma.
Peripheral blood samples were collected from Japanese patients with primary open-angle glaucoma (PG) (n = 206), exfoliation syndrome (EX) (n = 199), and controls (n = 126). Plasma levels of lipid peroxides, ferric-reducing activity, and thiol antioxidant activity were measured by diacron reactive oxygen metabolites (dROM), biological antioxidant potential (BAP), and sulfhydryl (SH) tests, respectively, using a free radical analyzer.
In the PG, EX, and control groups, the mean ± standard deviation values were 355±63, 357±69, and 348±56 (U. Carr), respectively, for dROM; 1,951±282, 1,969±252, and 2,033±252 (µmol/L), respectively, for BAP (µmol/L); and 614±98, 584±91, and 617±99 (µmol/L), respectively, for SH. The differences in the BAP values were significant between the PG and control groups (p = 0.0062), for SH between the EX and control groups (p = 0.0017), and for SH between the PG and EX groups (p = 0.0026). After adjustment for differences in age and sex among groups using multiple regression analysis, lower BAP values were correlated significantly with PG (p = 0.0155) and EX (p = 0.0049). Higher dROM values with and without glaucoma were correlated with female gender, and lower SH values with older age. There were no significant differences between the higher (≥21 mmHg) and lower (<21 mmHg) baseline IOPs in the PG group or between the presence or absence of glaucoma in the EX group.
Lower systemic antioxidant capacity that measured by ferric-reducing activity is involved in the pathogenesis of PG and EX.
局部和全身氧化应激在眼压（IOP）升高和视神经损伤中的作用在青光眼的发病机制中被假设。为了验证这一点，我们通过分析青光眼患者的血液生化指标来测量系统中促氧化剂和抗氧化剂的水平。
从日本原发性开角型青光眼（PG）（n=206）、剥脱综合征（EX）（n=199）和对照组（n=126）患者中采集外周血样本。使用自由基分析仪，分别通过二氯代过氧化物反应性氧代谢物（dROM）、生物抗氧化能力（BAP）和巯基（SH）试验测量血浆脂质过氧化物、铁还原活性和巯基抗氧化活性水平。
在 PG、EX 和对照组中，dROM 的平均值±标准差分别为 355±63、357±69 和 348±56（U. Carr）；BAP（μmol/L）的平均值±标准差分别为 1,951±282、1,969±252 和 2,033±252（μmol/L）；SH 的平均值±标准差分别为 614±98、584±91 和 617±99（μmol/L）。PG 组和对照组之间的 BAP 值差异显著（p=0.0062），EX 组和对照组之间的 SH 值差异显著（p=0.0017），PG 组和 EX 组之间的 SH 值差异显著（p=0.0026）。使用多元回归分析调整组间年龄和性别差异后，较低的 BAP 值与 PG（p=0.0155）和 EX（p=0.0049）显著相关。无论是否患有青光眼，较高的 dROM 值都与女性性别相关，而较低的 SH 值与年龄较大相关。PG 组较高（≥21mmHg）和较低（<21mmHg）基线 IOP 之间以及 EX 组是否存在青光眼之间均无显著差异。
通过铁还原活性测量的系统抗氧化能力降低与 PG 和 EX 的发病机制有关。
