对原始临床样本中流感（H3N2）病毒的血凝素（HA）基因进行直接测序，结果显示其与在哺乳动物细胞中培养的病毒具有序列同一性。
Direct sequencing of the HA gene of influenza (H3N2) virus in original clinical samples reveals sequence identity with mammalian cell-grown virus.
作者信息
Katz J M, Wang M, Webster R G
机构信息
Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 38101.
出版信息
J Virol. 1990 Apr;64(4):1808-11. doi: 10.1128/JVI.64.4.1808-1811.1990.
Abstract
When influenza (H3N2) viruses from infected individuals are grown in embryonated chicken eggs, viruses are isolated which differ antigenically and structurally from viruses grown in mammalian Madin-Darby canine kidney (MDCK) cell culture [G.C. Schild, J.S. Oxford, J.C. de Jong, and R.G. Webster, Nature (London) 303:706-709, 1983]. To determine which of these viruses is most representative of virus replicating in the infected individual, a region of the HA gene of virus present in original clinical samples was amplified by using the polymerase chain reaction and sequenced directly. Comparison of 170 amino acid residues of HA1 flanking and containing the receptor-binding site and antigenic sites indicated that over this region, the HA of virus replicating in the infected individual was identical to that of virus after growth in MDCK cells and was distinct from the HA of viruses grown in eggs. Therefore, cultivation of human influenza H3N2 virus in mammalian MDCK cells results in a virus similar to the predominant population of virus found in the infected individual.
摘要
当从受感染个体分离出的甲型流感病毒（H3N2）株在鸡胚中培养时，所分离出的病毒在抗原性和结构上与在哺乳动物来源的犬肾传代细胞（MDCK）中培养的病毒不同[G.C. 希尔德、J.S. 牛津、J.C. 德容和R.G. 韦伯斯特，《自然》（伦敦）303:706 - 709，1983]。为了确定这些病毒中哪一种最能代表在受感染个体中复制的病毒，利用聚合酶链反应对原始临床样本中病毒的血凝素（HA）基因区域进行扩增并直接测序。对HA1中位于受体结合位点和抗原位点两侧并包含这些位点的170个氨基酸残基进行比较，结果表明，在该区域，在受感染个体中复制的病毒的HA与在MDCK细胞中培养后的病毒的HA相同，与在鸡胚中培养的病毒的HA不同。因此，人甲型流感H3N2病毒在哺乳动物MDCK细胞中培养会产生一种与在受感染个体中发现的主要病毒群体相似的病毒。
相似文献
1
Direct sequencing of the HA gene of influenza (H3N2) virus in original clinical samples reveals sequence identity with mammalian cell-grown virus.对原始临床样本中流感（H3N2）病毒的血凝素（HA）基因进行直接测序，结果显示其与在哺乳动物细胞中培养的病毒具有序列同一性。
J Virol. 1990 Apr;64(4):1808-11. doi: 10.1128/JVI.64.4.1808-1811.1990.
2
Amino acid sequence identity between the HA1 of influenza A (H3N2) viruses grown in mammalian and primary chick kidney cells.在哺乳动物细胞和原代鸡肾细胞中培养的甲型流感病毒（H3N2）的血凝素1（HA1）之间的氨基酸序列同一性。
J Gen Virol. 1992 May;73 ( Pt 5):1159-65. doi: 10.1099/0022-1317-73-5-1159.
3
Antigenic and structural characterization of multiple subpopulations of H3N2 influenza virus from an individual.来自个体的H3N2流感病毒多个亚群的抗原性和结构特征
Virology. 1988 Aug;165(2):446-56. doi: 10.1016/0042-6822(88)90588-0.
4
Host cell-mediated variation in H3N2 influenza viruses.宿主细胞介导的H3N2流感病毒变异
Virology. 1987 Feb;156(2):386-95. doi: 10.1016/0042-6822(87)90418-1.
5
Comparison of 10 influenza A (H1N1 and H3N2) haemagglutinin sequences obtained directly from clinical specimens to those of MDCK cell- and egg-grown viruses.直接从临床标本中获得的10个甲型流感病毒（H1N1和H3N2）血凝素序列与在MDCK细胞和鸡胚中培养的病毒的血凝素序列的比较。
J Gen Virol. 1993 Nov;74 ( Pt 11):2513-8. doi: 10.1099/0022-1317-74-11-2513.
6
Direct sequence determination of the influenza B HA-1 gene after PCR amplification of clinical specimens from an infected volunteer.
J Virol Methods. 1993 Sep;44(1):35-44. doi: 10.1016/0166-0934(93)90005-c.
7
Receptor-binding properties of modern human influenza viruses primarily isolated in Vero and MDCK cells and chicken embryonated eggs.主要在Vero细胞、MDCK细胞和鸡胚中分离得到的现代人流感病毒的受体结合特性。
Virology. 2003 Sep 1;313(2):473-80. doi: 10.1016/s0042-6822(03)00377-5.
8
Improvement of influenza A/Fujian/411/02 (H3N2) virus growth in embryonated chicken eggs by balancing the hemagglutinin and neuraminidase activities, using reverse genetics.通过反向遗传学平衡血凝素和神经氨酸酶活性来提高甲型流感病毒/福建/411/02（H3N2）在鸡胚中的生长。
J Virol. 2005 Jun;79(11):6763-71. doi: 10.1128/JVI.79.11.6763-6771.2005.
9
Structural and evolutionary characteristics of HA, NA, NS and M genes of clinical influenza A/H3N2 viruses passaged in human and canine cells.在人源和犬源细胞中传代的临床甲型H3N2流感病毒的血凝素（HA）、神经氨酸酶（NA）、非结构蛋白（NS）和基质蛋白（M）基因的结构与进化特征
J Clin Virol. 2009 Aug;45(4):322-33. doi: 10.1016/j.jcv.2009.05.030.
10
Sequence analysis of the haemagglutinin (HA) of influenza A (H1N1) viruses present in clinical material and comparison with the HA of laboratory-derived virus.对临床样本中甲型流感病毒（H1N1）血凝素（HA）进行序列分析，并与实验室衍生病毒的HA进行比较。
J Gen Virol. 1991 Nov;72 ( Pt 11):2671-7. doi: 10.1099/0022-1317-72-11-2671.
引用本文的文献
1
Immunogenicity and protection of recombinant self-assembling ferritin-hemagglutinin nanoparticle influenza vaccine in mice.重组自组装铁蛋白-血凝素纳米颗粒流感疫苗在小鼠体内的免疫原性及保护作用
Clin Exp Vaccine Res. 2025 Jan;14(1):23-34. doi: 10.7774/cevr.2025.14.e7. Epub 2025 Jan 17.
2
Hybrid-Capture Target Enrichment in Human Pathogens: Identification, Evolution, Biosurveillance, and Genomic Epidemiology.人类病原体中的杂交捕获目标富集：鉴定、进化、生物监测和基因组流行病学
Pathogens. 2024 Mar 23;13(4):275. doi: 10.3390/pathogens13040275.
3
The Epidemiology of Influenza and the Associated Vaccines Development in China: A Review.中国流感流行病学及相关疫苗研发综述
Vaccines (Basel). 2022 Nov 6;10(11):1873. doi: 10.3390/vaccines10111873.
4
Identification and differential expression of microRNAs in Madin-Darby canine kidney cells with high and low tumorigenicities.具有高致瘤性和低致瘤性的犬肾传代细胞系中微小RNA的鉴定与差异表达
Genes Genomics. 2022 Feb;44(2):187-196. doi: 10.1007/s13258-021-01177-x. Epub 2022 Jan 20.
5
Microfluidic sample preparation for respiratory virus detection: A review.用于呼吸道病毒检测的微流控样品制备：综述
Biomicrofluidics. 2021 Feb 11;15(1):011503. doi: 10.1063/5.0041089. eCollection 2021 Jan.
6
Comparison of antigenic mutation during egg and cell passage cultivation of H3N2 influenza virus.H3N2流感病毒在鸡胚和细胞传代培养过程中的抗原性变异比较。
Clin Exp Vaccine Res. 2020 Jan;9(1):56-63. doi: 10.7774/cevr.2020.9.1.56. Epub 2020 Jan 31.
7
Repeat vaccination reduces antibody affinity maturation across different influenza vaccine platforms in humans.重复接种会降低人类不同流感疫苗平台的抗体亲和力成熟。
Nat Commun. 2019 Jul 26;10(1):3338. doi: 10.1038/s41467-019-11296-5.
8
Protein Microarray Analysis of the Specificity and Cross-Reactivity of Influenza Virus Hemagglutinin-Specific Antibodies.蛋白质微阵列分析流感病毒血凝素特异性抗体的特异性和交叉反应性。
mSphere. 2018 Dec 12;3(6):e00592-18. doi: 10.1128/mSphere.00592-18.
9
Sequence amplification via cell passaging creates spurious signals of positive adaptation in influenza virus H3N2 hemagglutinin.通过细胞传代进行序列扩增会在甲型流感病毒H3N2血凝素中产生适应性增强的假阳性信号。
Virus Evol. 2016 Jul;2(2). doi: 10.1093/ve/vew026. Epub 2016 Oct 3.
10
Advances in novel influenza vaccines: a patent review.新型流感疫苗的进展：一项专利综述。
J Microbiol. 2016 Jun;54(6):403-12. doi: 10.1007/s12275-016-6176-7. Epub 2016 May 27.
本文引用的文献
1
Electrophoretic analysis of iodine-labeled influenza virus RNA segments.碘标记流感病毒RNA片段的电泳分析
Anal Biochem. 1980 Feb;102(1):228-32. doi: 10.1016/0003-2697(80)90343-7.
2
Evidence for host-cell selection of influenza virus antigenic variants.流感病毒抗原变异体宿主细胞选择的证据。
Nature. 1983;303(5919):706-9. doi: 10.1038/303706a0.
3
Alterations in the hemagglutinin associated with adaptation of influenza B virus to growth in eggs.与乙型流感病毒适应在鸡蛋中生长相关的血凝素改变。
Virology. 1985 May;143(1):166-74. doi: 10.1016/0042-6822(85)90105-9.
4
Host cell-mediated variation in H3N2 influenza viruses.宿主细胞介导的H3N2流感病毒变异
Virology. 1987 Feb;156(2):386-95. doi: 10.1016/0042-6822(87)90418-1.
5
Structural changes in the haemagglutinin which accompany egg adaptation of an influenza A(H1N1) virus.甲型H1N1流感病毒适应鸡胚过程中血凝素的结构变化
Virology. 1987 Sep;160(1):31-7. doi: 10.1016/0042-6822(87)90040-7.
6
Serological studies with influenza A(H1N1) viruses cultivated in eggs or in a canine kidney cell line (MDCK).对在鸡蛋中或犬肾细胞系（MDCK）中培养的甲型H1N1流感病毒进行的血清学研究。
Bull World Health Organ. 1987;65(2):181-7.
7
Antigenic and structural characterization of multiple subpopulations of H3N2 influenza virus from an individual.来自个体的H3N2流感病毒多个亚群的抗原性和结构特征
Virology. 1988 Aug;165(2):446-56. doi: 10.1016/0042-6822(88)90588-0.
8
Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.用于镰状细胞贫血诊断的β-珠蛋白基因组序列的酶促扩增及限制性酶切位点分析。
Science. 1985 Dec 20;230(4732):1350-4. doi: 10.1126/science.2999980.
9
Host cell-specific growth advantage of pseudorabies virus with a deletion in the genome sequences encoding a structural glycoprotein.在编码一种结构糖蛋白的基因组序列中存在缺失的伪狂犬病病毒的宿主细胞特异性生长优势。
J Virol. 1988 Jan;62(1):12-9. doi: 10.1128/JVI.62.1.12-19.1988.
10
Efficacy of inactivated influenza A virus (H3N2) vaccines grown in mammalian cells or embryonated eggs.在哺乳动物细胞或鸡胚中培养的甲型流感病毒（H3N2）灭活疫苗的效力
J Infect Dis. 1989 Aug;160(2):191-8. doi: 10.1093/infdis/160.2.191.
Zotero 插件