Katz J M, Webster R G
Department of Virology/Molecular Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 38101.
J Infect Dis. 1989 Aug;160(2):191-8. doi: 10.1093/infdis/160.2.191.
Influenza virus (H3N2) host cell variants isolated from a single infected individual were compared for their protective efficacies when used as formalin-inactivated purified whole virus vaccines in ferrets. A/Mem/12/85 virus grown in embryonated chicken eggs (egg-grown), which differs from A/Mem/12/85 grown in mammalian Madin-Darby canine kidney cells (MDCK-grown) by a single amino acid substitution in the hemagglutinin molecule, was shown to be distinguishable by immune ferret serum. Ferrets were immunized intramuscularly with intact inactivated MDCK- or egg-grown virus and were subsequently challenged with infectious virus grown in either host cell type. MDCK-grown-virus vaccine induced higher mean serum hemagglutination-inhibiting (HAI) and neutralizing antibody titers than did egg-grown-virus vaccine and induced superior protection of ferrets against subsequent challenge with infectious virus grown in either host cell type. These results suggest that human influenza viruses that are antigenically and structurally similar to viruses grown in mammalian cells may be more efficacious as vaccines than some variants selected in eggs.
从单个感染个体中分离出的甲型流感病毒（H3N2）宿主细胞变体，作为福尔马林灭活的纯化全病毒疫苗用于雪貂时，对其保护效力进行了比较。在鸡胚中培养的A/Mem/12/85病毒（鸡胚培养），其血凝素分子中有一个氨基酸取代，与在哺乳动物马-达二氏犬肾细胞（MDCK培养）中培养的A/Mem/12/85病毒不同，免疫雪貂血清可将两者区分开来。雪貂通过肌肉注射完整的灭活MDCK培养或鸡胚培养病毒进行免疫，随后用在任一宿主细胞类型中培养的感染性病毒进行攻击。与鸡胚培养病毒疫苗相比，MDCK培养病毒疫苗诱导的平均血清血凝抑制（HAI）和中和抗体滴度更高，并且能为雪貂提供更好的保护，使其免受在任一宿主细胞类型中培养的感染性病毒的后续攻击。这些结果表明，与在哺乳动物细胞中培养的病毒在抗原性和结构上相似的人流感病毒作为疫苗可能比在鸡蛋中选择的某些变体更有效。
