Department of Genetic Engineering, SRM University, Kattankulathur, 603203, Tamil Nadu, India.
Neurochem Res. 2013 Mar;38(3):486-93. doi: 10.1007/s11064-012-0940-9. Epub 2012 Dec 2.
Oxidative stress is a major player in aging and neurodegenerative disorders. Macromolecular damage occurs as a result of oxidative stress that affects the mitochondria. Mitochondrial damage leads to cell death by apoptosis or necrosis. EGCG is a tea polyphenol that protects the cells against oxidative stress. Neuroprotective potential of EGCG was tested against H(2)O(2) induced oxidative stress in PC-12 cells. PC-12 cells were grown in tissue culture flasks. Oxidative stress was induced by adding H(2)O(2) to the cells. EGCG was also added and the cell death was assessed using MTT assay. Oxidative stress was assessed by protein carbonyl and thiol status. Mitochondrial membrane potential was studied using JC-1 staining. TNF-α levels were assessed using ELISA. H(2)O(2) increased the protein carbonyl content and reduced the thiol status in the PC-12 cells. Cell death was increased in H(2)O(2) treated cells as shown by MTT assay. Mitochondrial membrane potential was also decreased along with increase in TNF-α level in H(2)O(2) treated cells. EGCG brought about an increase in the cellular thiol status and decreased the protein carbonyl content in the PC-12 cells. Cell death was attenuated by EGCG treatment along with an increase in mitochondrial membrane potential and decrease in TNF-α level. EGCG conferred its antioxidant potential to PC-12 cells as evident by decreased protein damage. Mitochondrial membrane potential was improved along with a decrement in the cell death in PC-12 cells. EGCG acts as a good neutraceutical antioxidant to render neuroprotectivity to PC-12 cells.
氧化应激是衰老和神经退行性疾病的主要参与者。氧化应激会导致大分子损伤，影响线粒体。线粒体损伤会导致细胞通过细胞凋亡或坏死而死亡。EGCG 是一种茶多酚，可保护细胞免受氧化应激。用 H（2）O（2）诱导 PC-12 细胞的氧化应激来测试 EGCG 的神经保护潜力。将 PC-12 细胞在组织培养瓶中生长。通过向细胞中添加 H（2）O（2）来诱导氧化应激。还添加了 EGCG，并使用 MTT 测定法评估细胞死亡。通过蛋白质羰基和巯基状态评估氧化应激。使用 JC-1 染色研究线粒体膜电位。使用 ELISA 评估 TNF-α 水平。H（2）O（2）增加了 PC-12 细胞中的蛋白质羰基含量并降低了巯基状态。如 MTT 测定所示，H（2）O（2）处理的细胞中细胞死亡增加。线粒体膜电位也随着 H（2）O（2）处理细胞中 TNF-α 水平的增加而降低。EGCG 使 PC-12 细胞中的细胞巯基状态增加，蛋白质羰基含量降低。EGCG 处理减轻了细胞死亡，同时增加了线粒体膜电位并降低了 TNF-α 水平。EGCG 赋予 PC-12 细胞抗氧化潜力，这从蛋白质损伤减少中可以看出。线粒体膜电位得到改善，同时 PC-12 细胞中的细胞死亡减少。EGCG 作为一种良好的营养抗氧化剂，可使 PC-12 细胞具有神经保护作用。
