Shenzhen Academy of Metrology and Quality Inspection , Shenzhen 518102, People's Republic of China.
J Agric Food Chem. 2012 Dec 26;60(51):12477-81. doi: 10.1021/jf303533p. Epub 2012 Dec 14.
Edible bird's nest (EBN) is made of the swiftlets' saliva, which has attracted rather more attention owing to its nutritious and medical properties. Although protein constitutes the main composition and plays an important role in EBN, few studies have focused on the proteomic profile of EBN. The purpose of this study was to produce a proteomic map and clarify common EBN proteins. Liquid-phase isoelectric focusing (LIEF) was combined with two-dimensional electrophoresis (2-DE) for comprehensive analysis of EBN proteins. From 20 to 100 protein spots were detected on 2-DE maps of EBN samples from 15 different sources. The proteins were mainly distributed in four taxa (A, B, C, and D) according to their molecular mass. Taxa A and D both contained common proteins and proteins that may be considered another characteristic of EBN. Taxon A was identified using MALDI-TOF-TOF/MS and found to be homologous to acidic mammalian chitinase-like ( Meleagris gallopavo ), which is in glycosyl hydrolase family 18.
燕窝由雨燕科金丝燕的唾液筑成，具有较高的营养价值和药用价值，因此备受关注。尽管蛋白质是燕窝的主要成分，在燕窝中发挥着重要作用，但目前有关燕窝蛋白质组学的研究还较少。本研究旨在制作一张蛋白质组图谱，阐明燕窝的常见蛋白质。采用液相等电聚焦（LIEF）与二维电泳（2-DE）相结合的方法，对 15 个不同来源的燕窝样品进行全面分析。在燕窝 2-DE 图谱上检测到 20 到 100 个蛋白斑点，这些蛋白主要根据分子质量分布在四个分类群（A、B、C 和 D）中。分类群 A 和 D 均含有共同的蛋白和可能被视为燕窝另一特征的蛋白。分类群 A 通过 MALDI-TOF-TOF/MS 鉴定，与酸性哺乳动物几丁质酶样（Meleagris gallopavo）同源，属于糖苷水解酶家族 18。
