Chattopadhyay S K, Hartley J W, Lander M R, Kramer B S, Rowe W P
J Virol. 1978 Apr;26(1):29-39. doi: 10.1128/JVI.26.1.29-39.1978.
The recently described amphotropic group of murine leukemia viruses constitutes a distinct biological group, differing from the ecotropic and xenotropic groups in host range, cross interference, and serological reactivity. Viruses of this group have been detected only in wild mice from certain areas in California. By using a [3H]DNA probe synthesized in an endogenous reaction from detergent-lysed amphotropic virus (strain 1504-A), it was demonstrated that the amphotropic murine leukemia viruses are distinct biochemically, in that 20% of the viral genome sequences are not shared by AKR-type ecotropic or nay of three types of xenotropic murine leukemia virus tested. A subset of these amphotropic unique sequences, comprising one half of them, is present in the genome of wild mouse ecotropic viruses and in Moloney and Rauscher viruses as well. Sequences homologous to the entire genome of 1504-A amphotropic virus are present in the cellular DNA of all eight inbred mouse strains tested, as well as in wild Mus in Asia, in amounts varying from three to six complete viral genomes per haploid cell genome. Evidence is presented that at least 20% of the DNA sequences in both mouse- and mink-grown murine leukemia virus probes are of host-cell origin.
最近描述的嗜双性小鼠白血病病毒组构成一个独特的生物学类群，在宿主范围、交叉干扰和血清学反应性方面不同于亲嗜性和异嗜性病毒组。该组病毒仅在加利福尼亚某些地区的野生小鼠中检测到。通过使用在去污剂裂解的嗜双性病毒（1504 - A株）的内源反应中合成的[3H]DNA探针，证明嗜双性小鼠白血病病毒在生化方面是独特的，因为20%的病毒基因组序列与测试的AKR型亲嗜性病毒或三种异嗜性小鼠白血病病毒中的任何一种都不相同。这些嗜双性独特序列的一个子集，占其中一半，也存在于野生小鼠亲嗜性病毒以及莫洛尼病毒和劳舍尔病毒的基因组中。与1504 - A嗜双性病毒的整个基因组同源的序列存在于所测试的所有八个近交小鼠品系的细胞DNA中，以及亚洲的野生小家鼠中，每个单倍体细胞基因组中完整病毒基因组的数量从三个到六个不等。有证据表明，在小鼠和貂生长的小鼠白血病病毒探针中，至少20%的DNA序列来自宿主细胞。
