小家鼠脑池内A颗粒的遗传个体性。
Genetic individuality of intracisternal A-particles of Mus musculus.
作者信息
Lueders K K, Kuff E L
出版信息
J Virol. 1979 Apr;30(1):225-31. doi: 10.1128/JVI.30.1.225-231.1979.
Abstract
The nucleic acid sequence relationship between mouse intracisternal type A-particles and type C and B viruses was examined by reciprocal complementary DNA-RNA hybridization; complementary DNAs prepared from the RNAs of intracisternal A-particles were hybridized with high-molecular-weight RNAs from a variety of murine tumor viruses, and complementary DNAs representing a variety of RNA tumor virus genomes were hybridized with the high-molecular-weight RNAs from A-particles. The criterion for homology between two types of virus was that the heterologous hybridization reaction occurs over the same RNA concentration range as the homologous reacton. The results of these hybridizations indicate that there are no major sequence homologies between the RNA of intracisternal A-particles and the RNA of representative members of type B and C viruses of Mus musculus.
摘要
通过相互的互补DNA-RNA杂交来检测小鼠脑内A型颗粒与C型和B型病毒之间的核酸序列关系；从脑内A型颗粒RNA制备的互补DNA与多种鼠肿瘤病毒的高分子量RNA杂交，而代表多种RNA肿瘤病毒基因组的互补DNA则与A型颗粒的高分子量RNA杂交。两种病毒之间同源性的标准是，异源杂交反应与同源反应在相同的RNA浓度范围内发生。这些杂交结果表明，小鼠脑内A型颗粒的RNA与小家鼠B型和C型病毒代表性成员的RNA之间不存在主要的序列同源性。
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