Department of Respiratory Medicine, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou, China.
Epidemiol Infect. 2013 Oct;141(10):2158-62. doi: 10.1017/S0950268812002919. Epub 2012 Dec 10.
We investigated the virulence gene carriage and molecular type characteristics of Staphylococcus aureus isolates from bloodstream infections (BSIs) and skin and soft tissue infections (SSTIs) in children. A total of 71 isolates, 16 of which were methicillin-resistant S. aureus (MRSA), were investigated by PCR for virulence-associated gene profiles, sequence type and spa type. This revealed that 76.7% and 53.7% of the SSTI and BSI isolates, respectively, exhibited simultaneous carriage of o10 virulence genes. Compared to BSI isolates, carriage rates for hla, hlb, cna, clfA, seb, sec and pvl genes were significantly higher in SSTI isolates. By contrast, carriage of eta, etb and sea was significantly higher for BSI isolates. Thirty-four sequence types (STs) and 36 spa types were identified in the 71 isolates and included 14 novel STs and four novel spa types. ST59-MRSA-IV/V-t437 was the most common clone in the MRSA isolates. We concluded that virulence determinants are widely distributed in isolates of S. aureus strains from children with BSIs and SSTIs, with an unexpectedly high rate in SSTI isolates. Future profiling of S. aureus virulence determinants may allow the prediction of severity and outcome for children with these infections.
我们调查了儿童血流感染（BSI）和皮肤软组织感染（SSTI）中金黄色葡萄球菌分离株的毒力基因携带情况和分子型特征。通过 PCR 对 71 株分离株的毒力相关基因谱、序列型和 spa 型进行了研究，其中 16 株为耐甲氧西林金黄色葡萄球菌（MRSA）。结果显示，分别有 76.7%和 53.7%的 SSTI 和 BSI 分离株同时携带 o10 毒力基因。与 BSI 分离株相比，SSTI 分离株携带 hla、hlb、cna、clfA、seb、sec 和 pvl 基因的比率显著更高。相反，eta、etb 和 sea 基因在 BSI 分离株中的携带率更高。71 株分离株共鉴定出 34 种序列型（ST）和 36 种 spa 型，包括 14 种新型 ST 和 4 种新型 spa 型。MRSA 分离株中最常见的克隆是 ST59-MRSA-IV/V-t437。我们的结论是，毒力决定因素广泛存在于儿童 BSI 和 SSTI 金黄色葡萄球菌分离株中，SSTI 分离株中的携带率出乎意料地高。未来对金黄色葡萄球菌毒力决定因素的分析可能有助于预测这些感染患儿的严重程度和结局。
