Department of Phytochemistry, Regional Medical Research Centre, Indian Council of Medical Research, Belgaum, Karnataka 590010, India.
J Ethnopharmacol. 2013 Jan 30;145(2):621-5. doi: 10.1016/j.jep.2012.11.045. Epub 2012 Dec 7.
Cyathocline purpurea (D. Don.) O. Ktze. (Asteraceae) is a rare existence Indian medicinal plant and traditionally has antimicrobial property.
The aim of the present study was to identify chemical composition of the essential oil from the roots of Cyathocline purpurea and to screened in vitro antibacterial activity against eight human pathogenic bacteria.
The essential oil of roots was analyzed by using GC-FID and GC-MS. The antibacterial activity of oil was tested against four Gram-positive and four Gram-negative bacteria and antibacterial activity was determined by the tube dilution method.
The main constituents of the oil were thymohydroquinone dimethyl ether (57.4%) and β-selinene (14.0%), among twenty five identified compounds, which represented 90.1% of the total oil. The oil was found active against Gram-positive bacteria with minimal bactericidal concentration (MBC) values in the range of 0.26-0.57 mg/mL.
This is the first report on the chemical composition and antibacterial activity of the essential oil of roots of Cyathocline purpurea. The observation of MBC assay suggested that the Gram positive microorganisms were susceptible to essential oil, while oil was found to be resistant against Gram-negative bacteria, and the oil has bactericidal property.
紫菀木（D. Don.）O. Ktze.（菊科）是一种罕见的印度药用植物，传统上具有抗菌特性。
本研究的目的是鉴定紫菀木根的精油的化学成分，并筛选其对八种人体病原菌的体外抗菌活性。
采用 GC-FID 和 GC-MS 分析根的精油。通过管稀释法测试油对四种革兰氏阳性菌和四种革兰氏阴性菌的抗菌活性。
该油的主要成分是百里香氢醌二甲醚（57.4%）和β-瑟林烯（14.0%），在鉴定的二十五种化合物中，占总油量的 90.1%。该油对革兰氏阳性菌具有活性，最小杀菌浓度（MBC）值在 0.26-0.57mg/mL 范围内。
这是首次报道紫菀木根精油的化学成分和抗菌活性。MBC 测定的观察结果表明，革兰氏阳性微生物对精油敏感，而油对革兰氏阴性菌有耐药性，并且油具有杀菌特性。
