Environnements et Paléoenvironnements Océaniques et Continentaux (EPOC, UMR 5805 CNRS), Laboratoire de Physico- et Toxico-Chimie de l'Environnement (LPTC), Université de Bordeaux, 351 cours de la Libération, 33405 Talence cedex, France.
Sci Total Environ. 2013 Jan 15;443:621-32. doi: 10.1016/j.scitotenv.2012.10.015. Epub 2012 Dec 7.
The aim of this work was to investigate the potential presence of a broad range of organic compounds, such as hormones, alkylphenols, bisphenol A and phthalates, as well as pharmaceutical substances in two brands of bottled natural mineral waters (Evian and Volvic, Danone). The phthalates were determined by solid-phase microextraction coupled to gas chromatography-mass spectrometry (SPME-GC-MS) and the other compounds by liquid chromatography-tandem mass spectrometry (LC-MS/MS) or gas chromatography-mass spectrometry (GC-MS) after solid-phase extraction. The potential migration of alkylphenols, bisphenol A and phthalates from polyethylene terephthalate (PET) bottles was also investigated under standardized test conditions. Evian and Volvic natural mineral waters contain none of the around 120 targeted organic compounds. Traces of 3 pharmaceuticals (ketoprofen, salicylic acid, and caffeine), 3 alkylphenols (4-nonylphenol, 4-t-octylphenol, and 4-nonylphenol diethoxylate), and some phthalates including di(2-ethylhexyl)phthalate (DEHP) were detected in the samples, but they were also present in the procedural blanks at similar levels. The additional test procedures demonstrated that the few detected compounds originated from the background laboratory contamination. Analytical procedures have been designed both in the bottling factory and in the laboratory in order to investigate the sources of DEHP and to minimize to the maximum this unavoidable laboratory contamination. It was evidenced that no migration of the targeted compounds from bottles occurred under the test conditions. The results obtained in this study underline the complexity of reaching a reliable measure to qualify the contamination of a sample at ultra-trace level, in the field of very pure matrices. The analytical procedures involving glassware, equipment, hoods, and rooms specifically dedicated to trace analysis allowed us to reach reliable procedural limits of quantification at the ng/L level, by lowering the background laboratory contamination.
这项工作的目的是调查两种瓶装天然矿泉水（依云和沃维克，达能）中是否存在广泛的有机化合物，如激素、烷基酚、双酚 A 和邻苯二甲酸酯以及药物物质。采用固相微萃取结合气相色谱-质谱联用（SPME-GC-MS）测定邻苯二甲酸酯，采用固相萃取后液相色谱-串联质谱（LC-MS/MS）或气相色谱-质谱（GC-MS）测定其他化合物。还在标准化测试条件下研究了聚对苯二甲酸乙二醇酯（PET）瓶中烷基酚、双酚 A 和邻苯二甲酸酯的潜在迁移情况。依云和沃维克天然矿泉水不含约 120 种目标有机化合物。在样品中检测到 3 种药物（酮洛芬、水杨酸和咖啡因）、3 种烷基酚（4-壬基酚、4-叔辛基酚和 4-壬基酚二乙氧基化物）和一些邻苯二甲酸酯，包括邻苯二甲酸二（2-乙基己基）酯（DEHP），但在程序空白中也以相似水平存在。其他测试程序表明，检测到的少量化合物来自实验室背景污染。已在装瓶厂和实验室设计了分析程序，以调查 DEHP 的来源，并最大程度地减少这种不可避免的实验室污染。有证据表明，在测试条件下，目标化合物不会从瓶中迁移。本研究结果强调了在非常纯净的基质领域，达到可靠的测量方法来定性痕量水平样品污染的复杂性。涉及玻璃器皿、设备、通风橱和专门用于痕量分析的房间的分析程序允许我们通过降低实验室背景污染，将可靠的程序定量下限降低到 ng/L 水平。
