Research Group of Functional Genomics and Proteomics, KU Leuven, B-3000 Leuven, Belgium.
Peptides. 2013 Apr;42:63-9. doi: 10.1016/j.peptides.2012.11.018. Epub 2012 Dec 8.
Tear fluid is a complex mixture of biological compounds, including carbohydrates, lipids, electrolytes, proteins, and peptides. Despite the physiological importance of tear fluid, little is known about the identity of its endogenous peptides. In this study, we analyzed and identified naturally occurring peptide molecules in human reflex tear fluid by means of LC-MALDI-TOF-TOF. Tandem MS analyses revealed 30 peptides, most of which have not been identified before. Twenty-six peptides are derived from the proline-rich protein 4 and 4 peptides are derived from the polymeric immunoglobulin receptor. Based on their structural characteristics, we suggest that the identified tear fluid peptides contribute to the protective environment of the ocular surface.
泪液是一种复杂的生物化合物混合物，包括碳水化合物、脂质、电解质、蛋白质和肽。尽管泪液具有重要的生理意义，但对于其内源性肽的身份知之甚少。在这项研究中，我们通过 LC-MALDI-TOF-TOF 分析和鉴定了人反射性泪液中的天然存在的肽分子。串联 MS 分析揭示了 30 种肽，其中大多数以前未被鉴定过。26 种肽来源于富含脯氨酸的蛋白 4，4 种肽来源于多聚免疫球蛋白受体。根据它们的结构特征，我们认为鉴定出的泪液肽有助于眼表的保护环境。
