Getahun Abiey, Kifle Zemene Demelash, Ambikar Digambar, Atnafie Seyfe Asrade
Gondar University Specialized Hospital, University of Gondar, Gondar, Ethiopia.
Department of Pharmacology, School of Pharmacy, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.
Metabol Open. 2021 Jul 16;11:100110. doi: 10.1016/j.metop.2021.100110. eCollection 2021 Sep.
In diabetes mellitus, the wound healing process is impaired and delayed. Plants are actively investigated for safe, effective, and well-tolerated treatment options for wound. The plant has a traditional claim for the treatment of wound. Fresh leaves are crushed and given for topical application. The objective of this study was to evaluate the wound healing activity of hydro-alcoholic extract and solvent fractions of leaves of fresen (Buddlejaceae) in normal and diabetic mice.
leaves were extracted using 80% methanol and further fractionated using chloroform, ethyl acetate, and water. The wound healing activity was tested using excision, incision, and diabetic wound models. Area of wound contraction, time of epithelialization, and wound breaking strength were determined.
Treatment with 5% w/w (P < 0 0.05) and 10% w/w (P < 0.001) crude extract of significantly lowered wound area as compared to simple ointment. Treatment of excision wound with 5% w/w and 10% w/w of chloroform, ethyl acetate, and aqueous fractions of significantly lowered wound area and epithelization period (P < 0.05). The 10% w/w extract of showed a significant area of diabetic wound contraction compared to 5% w/w extract and simple ointment. The 10% w/w and 5% w/w extracts of significantly increased wound breaking strength compared to untreated and simple ointment groups (P < 0.05).
The crude and the solvent fractions of leaves possess wound healing activities as evidenced by an increase in tensile strength and wound contraction rate and decrease in the epithelialization period. This finding supports the traditional claims of for the healing of wounds.
在糖尿病中，伤口愈合过程受损且延迟。人们正在积极研究植物，以寻找安全、有效且耐受性良好的伤口治疗方案。这种植物在传统上就有治疗伤口的说法。将新鲜叶子捣碎后用于局部涂抹。本研究的目的是评估新鲜醉鱼草（醉鱼草科）叶子的水醇提取物和溶剂馏分在正常和糖尿病小鼠中的伤口愈合活性。
用80%甲醇提取叶子，再用氯仿、乙酸乙酯和水进一步分离。使用切除、切开和糖尿病伤口模型测试伤口愈合活性。测定伤口收缩面积、上皮化时间和伤口抗张强度。
与单纯软膏相比，用5% w/w（P < 0.05）和10% w/w（P < 0.001）的粗提取物处理显著降低了伤口面积。用5% w/w和10% w/w的氯仿、乙酸乙酯和水馏分处理切除伤口，显著降低了伤口面积和上皮化时间（P < 0.05）。与5% w/w提取物和单纯软膏相比，10% w/w的新鲜醉鱼草提取物显示出糖尿病伤口收缩的显著面积。与未处理组和单纯软膏组相比，10% w/w和5% w/w的新鲜醉鱼草提取物显著提高了伤口抗张强度（P < 0.05）。
新鲜醉鱼草叶子的粗提物和溶剂馏分具有伤口愈合活性，这通过抗张强度和伤口收缩率的增加以及上皮化时间的减少得到证明。这一发现支持了新鲜醉鱼草在伤口愈合方面的传统说法。
