Center for Ophthalmic Optics and Lasers, Casey Eye Institute and Department of Ophthalmology, Oregon Health and Science University, Portland, Oregon 97239, USA.
Invest Ophthalmol Vis Sci. 2013 Jan 9;54(1):258-65. doi: 10.1167/iovs.12-10477.
To quantify cells in the ocular anterior chamber (AC) by optical coherence tomography (OCT).
A time-domain anterior segment OCT system was used to image latex microsphere suspensions in vitro and the AC of uveitis and normal subjects in vivo. The OCT scan pattern, consisting of 2- and 4-mm-diameter concentric circular scans, was divided into central, superior, and inferior regions. A computer algorithm was developed to automatically identify particles in OCT images. A uveitis specialist used slit-lamp biomicroscopy to grade the AC cells on a scale of 0 to 4+.
Latex microspheres and ac cells were visualized as reflective spots in oct images. OCT latex microsphere concentration measurements were highly correlated to known particle concentrations (r = 1.000) and had an efficiency of 0.72. in 30 nongranulomatous and 12 granulomatous eyes, the OCT cell counts correlated well with slit-lamp grades in all three regions (Spearman's rho coefficient: >0.63). The average OCT cell count was 3.7 cells/grade in nongranulomatous eyes and 2.0 cells/grade in granulomatous eyes. OCT revealed significant amounts of inferior AC cells in 5 of 16 quiescent uveitis eyes (mean ± SD: 19.9 ± 7.4 cells). OCT captured rare cells in normal eyes (1.1 ± 1.1 cells centrally).
OCT provided quantitative information on AC inflammatory cells. The OCT cell counts correlated well with clinical grading, and particles in the inferior AC that were missed by slit-lamp examination were detected by OCT. OCT could be a valuable tool for the diagnosis and management of anterior uveitis.
利用光相干断层扫描（OCT）定量眼前房（AC）中的细胞。
使用时域眼前段 OCT 系统对体外乳胶微球悬浮液和葡萄膜炎及正常受试者的眼前房进行成像。OCT 扫描模式由 2mm 和 4mm 直径的同心圆形扫描组成，分为中央、上和下区域。开发了一种计算机算法来自动识别 OCT 图像中的颗粒。葡萄膜炎专家使用裂隙灯生物显微镜对眼前房细胞进行分级，范围为 0 至 4+。
乳胶微球和 AC 细胞在 OCT 图像中显示为反射斑点。OCT 乳胶微球浓度测量与已知颗粒浓度高度相关（r=1.000），效率为 0.72。在 30 只非肉芽肿性和 12 只肉芽肿性眼中，OCT 细胞计数与所有三个区域的裂隙灯分级均具有良好的相关性（Spearman's rho 系数：>0.63）。非肉芽肿性眼中的平均 OCT 细胞计数为 3.7 个/级，肉芽肿性眼中为 2.0 个/级。OCT 显示 16 只静止性葡萄膜炎眼中有 5 只（平均值±标准差：19.9±7.4 个）下眼前房 AC 细胞明显增多。OCT 在正常眼中捕捉到少量细胞（中央 1.1±1.1 个）。
OCT 提供了关于 AC 炎性细胞的定量信息。OCT 细胞计数与临床分级相关性良好，并且裂隙灯检查遗漏的下眼前房颗粒可通过 OCT 检测到。OCT 可能是诊断和管理前葡萄膜炎的有用工具。
