鬼臼根茎提取物的生化成分和抗氧化能力。

Biochemical composition and antioxidant capacity of extracts from Podophyllum hexandrum rhizome.

机构信息

Gansu Provincial Key Laboratory of Aridland Crop Science/College of Life Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, PR China.

出版信息

BMC Complement Altern Med. 2012 Dec 22;12:263. doi: 10.1186/1472-6882-12-263.

BACKGROUND

Podophyllum hexandrum Royle (P. hexandrum) is a perennial herb and widely used in clinic. The present study was designed to separate and identify the biochemical composition and antioxidant capacity of extracts from P. hexandrum rhizome.

METHODS

The ethyl acetate and ethanol extracts from P. hexandrum rhizome were analyzed by GC-MS (gas chromatography-mass spectrometry), and the antioxidant capacity of the extracts and the components was tested by using the DPPH (2, 2-diphenylpicrylhydrazyl) and FRAP (Ferric reducing/antioxidant power) assays.

RESULTS

The rhizome extracts had greater antioxidant capacity than the petiole extracts in DPPH and FRAP assays. About 16 kinds of main reactive oxygen components were identified in the extracts. Components of PADE (Phthalic acid, diisobutyl ester), BADE (1,2-Benzenedicarboxylic acid, diisooctyl ester), Polyneuridine, PODD (Podophyllotoxin, deoxy), β-Sitosterol and POD (Podophyllotoxin) showed the antioxidant capacity in some degree. PODD, POD, and Polyneuridine showed stronger antioxidant capacity with the IC50 and FRAP values of 9.61 ± 0.81 and 2923.98 ± 21.89 μM, 9.98 ± 0.24 and 2847.27 ± 14.82 μM, and 13.37 ± 0.35 and 2404.32 ± 36.88 μM, respectively, than the positive control ASA (Ascorbic acid) with the values of 60.78 ± 1.22 and 1267.5 ± 30.24 μM (P < 0.01).

CONCLUSIONS

PODD, POD, and Polyneuridine are very critical for the antioxidant capacity in the extract of P. hexandrum rhizome. These results provide useful biochemical basis and information for the potential use of this plant.

背景

鬼臼（Podophyllum hexandrum Royle）是一种多年生草本植物，广泛应用于临床。本研究旨在分离和鉴定鬼臼根茎提取物的生化成分和抗氧化能力。

方法

采用气相色谱-质谱联用（GC-MS）分析鬼臼根茎的乙酸乙酯和乙醇提取物，并通过 DPPH（2,2-二苯基-1-苦肼基）和 FRAP（铁还原/抗氧化能力）测定法测试提取物和成分的抗氧化能力。

结果

在 DPPH 和 FRAP 测定中，根茎提取物的抗氧化能力强于叶柄提取物。在提取物中鉴定出约 16 种主要活性氧成分。PADE（邻苯二甲酸二异丁酯）、BADE（1,2-苯二甲酸二异辛酯）、多神经啶、PODD（鬼臼毒素，脱氧）、β-谷甾醇和 POD（鬼臼毒素）等成分在一定程度上表现出抗氧化能力。PODD、POD 和多神经啶的 IC50 和 FRAP 值分别为 9.61 ± 0.81 和 2923.98 ± 21.89 μM、9.98 ± 0.24 和 2847.27 ± 14.82 μM、13.37 ± 0.35 和 2404.32 ± 36.88 μM，均强于阳性对照 ASA（抗坏血酸）的 60.78 ± 1.22 和 1267.5 ± 30.24 μM（P < 0.01）。