Institute of Biochemical Engineering, Technische Universität Braunschweig, Germany.
Bioresour Technol. 2013 May;135:544-54. doi: 10.1016/j.biortech.2012.11.047. Epub 2012 Nov 19.
Bio-based production promises a sustainable route to myriads of chemicals, materials and fuels. With regard to eco-efficiency, its future success strongly depends on a next level of bio-processes using raw materials beyond glucose. Such renewables, i.e., polymers, complex substrate mixtures and diluted waste streams, often cannot be metabolized naturally by the producing organisms. This particularly holds for well-known microorganisms from the traditional sugar-based biotechnology, including Escherichia coli, Corynebacterium glutamicum and Saccharomyces cerevisiae which have been engineered successfully to produce a broad range of products from glucose. In order to make full use of their production potential within the bio-refinery value chain, they have to be adapted to various feed-stocks of interest. This review focuses on the strategies to be applied for this purpose which combine rational and evolutive approaches. Hereby, the three industrial platform microorganisms, E. coli, C. glutamicum and S. cerevisiae are highlighted due to their particular importance.
生物基生产有望为无数化学品、材料和燃料提供可持续的途径。就生态效率而言，其未来的成功在很大程度上取决于下一阶段使用葡萄糖以外的原材料的生物工艺。这些可再生资源，如聚合物、复杂的基质混合物和稀释的废物流，通常不能被产生它们的生物体自然代谢。对于来自传统糖基生物技术的知名微生物，这一点尤其如此，包括大肠杆菌、谷氨酸棒状杆菌和酿酒酵母，它们已经被成功地工程化以从葡萄糖生产广泛的产品。为了在生物炼制价值链中充分利用它们的生产潜力，它们必须适应各种感兴趣的饲料。这篇综述重点介绍了为此目的而应用的策略，这些策略结合了理性和进化的方法。在这里，由于其特殊的重要性，突出了三种工业平台微生物，大肠杆菌、谷氨酸棒状杆菌和酿酒酵母。
