细胞培养中的甲型肝炎病毒。IV. 在诊断测试系统中，对细胞培养产生的甲型肝炎病毒与粪便来源的甲型肝炎病毒进行比较。
Hepatitis A-virus in cell culture. IV. Comparison of cell-culture-produced HAV with stool-derived HAV in diagnostic test systems.
作者信息
Flehmig B, Pfisterer M, Vallbracht A
出版信息
Med Microbiol Immunol. 1984;172(4):207-13. doi: 10.1007/BF02123715.
Hepatitis A-virus produced in Frhk-4/R cells is used and compared with stool-derived HAV as an antigen in diagnostic test systems. It is shown that the two antigens react identically in the anti-HAV test with anti-HAV-IgG positive human sera. In the anti-HAV-IgM test, too, stool-derived HAV and cell-culture-produced HAV react very similarly. The titres of anti-HAV-IgG and anti-HAV-IgM positive sera, obtained with stool and cell-culture HAV in quantitative antibody determinations, are shown to be identical. It is shown that the problem of HAV antigen production can now be solved by propagation of HAV with Frhk-4/R cells and that this antigen is very useful in diagnostic test systems.
使用在Frhk-4/R细胞中产生的甲型肝炎病毒，并将其与粪便来源的甲型肝炎病毒作为抗原在诊断测试系统中进行比较。结果表明，在抗甲型肝炎病毒检测中，这两种抗原与抗甲型肝炎病毒IgG阳性人血清的反应相同。在抗甲型肝炎病毒IgM检测中，粪便来源的甲型肝炎病毒和细胞培养产生的甲型肝炎病毒反应也非常相似。在定量抗体测定中，用粪便和细胞培养的甲型肝炎病毒获得的抗甲型肝炎病毒IgG和抗甲型肝炎病毒IgM阳性血清的滴度显示相同。结果表明，现在可以通过用Frhk-4/R细胞繁殖甲型肝炎病毒来解决甲型肝炎病毒抗原生产的问题，并且这种抗原在诊断测试系统中非常有用。
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