School of Pharmacy, Yantai University, 264005 Yantai, Shandong, PR China.
Phytomedicine. 2013 Feb 15;20(3-4):319-23. doi: 10.1016/j.phymed.2012.11.016. Epub 2012 Dec 24.
In the present study, we evaluated the anti-inflammatory effect of 4-methoxy-5- hydroxycanthin-6-one (CAN), a natural alkaloid isolated from Picrasma quassioides. CAN significantly inhibited the production of NO and the release of TNF-α induced by LPS in macrophage RAW 264.7. Western blot showed that CAN can downregulate the expression of iNOS protein. After oral administration, CAN (3, 9, and 27 mg/kg) reduced the development of carrageenan-induced paw edema and complete Freund's adjuvant (CFA)-induced chronic arthritis in rats. The observed results indicated that pre-treatment with CAN might be an effective therapeutic intervention against inflammatory diseases including chronic arthritis.
在本研究中，我们评估了从瓜馥木中分离得到的天然生物碱 4-甲氧基-5-羟基卡亭酮（CAN）的抗炎作用。CAN 显著抑制 LPS 诱导的巨噬细胞 RAW 264.7 中 NO 的产生和 TNF-α 的释放。Western blot 显示 CAN 可以下调 iNOS 蛋白的表达。口服给予 CAN（3、9 和 27 mg/kg）可减轻角叉菜胶诱导的大鼠足肿胀和完全弗氏佐剂（CFA）诱导的慢性关节炎的发展。观察结果表明，CAN 的预处理可能是治疗包括慢性关节炎在内的炎症性疾病的有效治疗干预措施。
