RIKEN Plant Science Center, 1-7-22 Suehiro, Tsurumi, Yokohama 230-0045, Japan.
Phytochemistry. 2013 Mar;87:30-8. doi: 10.1016/j.phytochem.2012.11.023. Epub 2012 Dec 29.
Cytokinin (CK) is a plant hormone that plays important regulatory roles in many aspects of plant growth and development. Although functions of CK and its biosynthesis pathway have been studied extensively, there is still no efficient biosynthesis inhibitor, which would be useful for studying CK from a chemical genetic approach. Here, CK biosynthesis inhibitor candidates were searched for using a systematic approach. In silico screening of candidates were carried out using genome-wide gene expression profiles and prediction of target sites using global CK accumulation profile analysis. As a result of these screenings, it was found that uniconazole, a well known inhibitor of cytochrome P450 monooxygenase, prevents the biosynthesis of trans-zeatin, and that its target is CYP735As in Arabidopsis.
细胞分裂素（CK）是一种植物激素，在植物生长和发育的许多方面发挥着重要的调节作用。尽管 CK 及其生物合成途径的功能已被广泛研究，但仍然没有有效的生物合成抑制剂，这对于从化学遗传角度研究 CK 是非常有用的。在这里，我们使用系统的方法来寻找 CK 生物合成抑制剂的候选物。通过全基因组基因表达谱的计算筛选和利用全球 CK 积累谱分析来预测靶位，进行候选物的计算筛选。作为这些筛选的结果，发现一种众所周知的细胞色素 P450 单加氧酶抑制剂——烯效唑，可阻止反式玉米素的生物合成，其靶标是拟南芥中的 CYP735As。
