Departments of Medicine and †Pediatrics, North Shore University Hospital-Cornell University Medical College, Manhasset, New York, U.S.A.; *The Free University of Berlin, Berlin, Germany; and the ‡Department of Medicine, University of Maryland, Baltimore, Maryland, U.S.A.
Inflamm Bowel Dis. 1996 Spring;2(1):16-26.
: Crohn's disease (CD) and ulcerative colitis (UC) are idiopathic inflammatory bowel diseases (IBD) that are characterized by chronic intestinal inflammation and are associated with abnormalities of peripheral and mucosal immune function. The aim of our study was to determine whether CD or UC is characterized by discrete profiles of intestinal lymphokine production. Total cellular RNA was isolated from biopsies of healthy controls and from patients with IBD. Messenger RNA transcript levels in biopsies were determined for interleukin-2 (IL-2), IL-4, IL-5, and interferon-γ (IFN-γ), using a quantitative reverse transcriptase polymerase chain reaction method. Compared with inflamed UC mucosa and controls, CD mucosal lesions contained higher IL-2 and IFN-γ mRNA (p < 0.05), which is consistent with a T-helper cell 1 (Th1)-like pattern. In UC, IL-5 mRNA content was higher in involved areas compared with controls (p < 0.05) and inflamed CD lesions (p < 0.05), suggestive of a Th2 pattern. We conclude that the intestinal mucosa of CD and UC have inflammatory responses characterized by discrete T-helper profiles of lymphokines. This strongly suggests that the immunopathogenesis of these two forms of IBD are different.
克罗恩病（CD）和溃疡性结肠炎（UC）是特发性炎症性肠病（IBD），其特征为慢性肠道炎症，并伴有外周和黏膜免疫功能异常。我们的研究旨在确定 CD 或 UC 是否具有离散的肠道淋巴因子产生特征。从健康对照者和 IBD 患者的活检组织中分离总细胞 RNA。使用定量逆转录聚合酶链反应方法，测定活检组织中白细胞介素-2（IL-2）、IL-4、IL-5 和干扰素-γ（IFN-γ）的信使 RNA 转录水平。与炎症性 UC 黏膜和对照相比，CD 黏膜病变中含有更高水平的 IL-2 和 IFN-γ mRNA（p < 0.05），这与 Th1 样模式一致。在 UC 中，受累区域的 IL-5 mRNA 含量高于对照（p < 0.05）和炎症性 CD 病变（p < 0.05），提示 Th2 模式。我们得出结论，CD 和 UC 的肠道黏膜具有以离散的辅助性 T 细胞淋巴因子特征为特征的炎症反应。这强烈表明这两种形式的 IBD 的免疫发病机制不同。
