新型核酮糖碳插入反应的钼辅因子生物合成酶 MoaA 的催化作用。
Catalysis of a new ribose carbon-insertion reaction by the molybdenum cofactor biosynthetic enzyme MoaA.
机构信息
Department of Chemistry, Texas A&M University, College Station, TX 77842, USA.
出版信息
Biochemistry. 2013 Feb 19;52(7):1134-6. doi: 10.1021/bi3016026. Epub 2013 Feb 4.
Abstract
MoaA, a radical S-adenosylmethionine enzyme, catalyzes the first step in molybdopterin biosynthesis. This reaction involves a complex rearrangement in which C8 of guanosine triphosphate is inserted between C2' and C3' of the ribose. This study identifies the site of initial hydrogen atom abstraction by the adenosyl radical and advances a mechanistic proposal for this unprecedented reaction.
摘要
MoaA 是一种激进的 S-腺苷甲硫氨酸酶，催化钼喋呤生物合成的第一步。该反应涉及一个复杂的重排过程，其中三磷酸鸟苷的 C8 插入核糖的 C2' 和 C3' 之间。本研究确定了腺苷自由基引发的初始氢原子的抽离位置，并提出了该前所未有的反应的机制建议。
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