Refik Saydam National Public Health Agency, Ankara, Turkey.
Vector Borne Zoonotic Dis. 2013 Feb;13(2):111-8. doi: 10.1089/vbz.2011.0939. Epub 2013 Jan 5.
This study was carried out to better understand the epidemiology of hantaviruses in a province of Turkey (Giresun) where human hantavirus disease has recently been detected. In this cross-sectional study, a total of 626 blood samples from healthy people aged 15 and 84 years old were collected both in urban and rural areas in 2009. The sera were tested by enzyme-linked immunosorbent assay (ELISA), immunoblotting assay, and the focus reduction neutralization test (FRNT). We screened the samples by an ELISA and found that 65/626 samples reacted positively for the presence of hantavirus-reactive immunoglobulin G (IgG). Twenty of the 65 ELISA-positive samples could be confirmed by an immunobloting assay, and the overall seroprevalence was thereby calculated to 3.2% (20/626). The seroprevalence of the people living in wood areas or adobe houses 9/17 (52.9%) was significantly higher than among people living in concrete houses 10/47 (21.3%) (p=0.014). Finally, 3 of the 20 immunoblot-positive sera were confirmed as specific for the Puumala hantavirus serotype by FRNT, 1 serum was confirmed as Dobrava virus-specific, whereas 1 serum was found to be equally reactive to Dobrava and Saaremaa viruses. We will now focus on further investigations of the ecology and epidemiology of hantaviruses in humans and their carrier animals in Turkey, studies that have already been started and will be further intensified.
本研究旨在更好地了解土耳其一个省（吉雷松省）的汉坦病毒流行病学情况，该省最近已检测到人类汉坦病毒病。在这项横断面研究中，我们于 2009 年在城乡共采集了 626 份年龄在 15 至 84 岁的健康人群的血液样本。血清通过酶联免疫吸附试验（ELISA）、免疫印迹试验和焦点减少中和试验（FRNT）进行检测。我们通过 ELISA 筛选了这些样本，发现 65/626 个样本对汉坦病毒反应性免疫球蛋白 G（IgG）呈阳性反应。在 65 个 ELISA 阳性样本中，有 20 个可通过免疫印迹试验得到确认，因此总体血清阳性率计算为 3.2%（20/626）。居住在木质或土坯房屋中的人群的血清阳性率为 9/17（52.9%），显著高于居住在混凝土房屋中的人群的血清阳性率 10/47（21.3%）（p=0.014）。最后，通过 FRNT，有 3 份免疫印迹阳性血清被确认为特异性的普马拉汉坦病毒血清型，1 份血清被确认为多布拉瓦病毒特异性，而 1 份血清对多布拉瓦和萨雷马病毒均具有同等反应性。我们现在将重点关注进一步调查汉坦病毒在人类及其载体动物中的生态和流行病学，这些研究已经开始，并将进一步加强。
