School of Fisheries and life, Shanghai Ocean University, Shanghai 201306, PR China.
Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources of China, State Key Laboratory Breeding Base of Marine Genetic Resources, Fujian Key Laboratory of Marine Genetic Resources, Xiamen 361005, PR China.
Int J Syst Evol Microbiol. 2022 Feb;72(2). doi: 10.1099/ijsem.0.005217.
Two novel Gram-stain-negative, aerobic, rod-shaped, carotenoid-pigmented and non-flagellated bacteria, designated BC31-1-A7 and BC31-3-A3, were isolated from polyethylene-terephthalate-degrading bacterial consortia enriched from deep-sea sediment collected in the Pacific Ocean. Optimal growth of both strains was observed at 28-32 °C, at pH 7.5 and in the presence of 3-4% (w/v) NaCl. The 16S rRNA gene sequence analysis revealed that strains BC31-1-A7 and BC31-3-A3 were closely related to JCM 11811, KCTC 22173, DSM 13258, DSM 25030, JCM 33902 and KCTC 72200 with 96.8-98.9% sequence similarity. The 16S rRNA gene sequence similarity between strains BC31-1-A7 and BC31-3-A3 was 97.5%. The genomic G+C contents of strains BC31-1-A7 and BC31-3-A3 were 42.1 and 41.6 mol%, respectively. The average nucleotide identity and digital DNA-DNA hybridization values between strain BC31-3-A3, strain BC31-1-A7 and their six closely related type strains were 77.6-84.3% and 20.5-27.9%, respectively. Menaquinone-6 was detected as the major isoprenoid quinone in all strains. Their major fatty acids were iso-C, iso-C G and iso-C 3-OH. The major polar lipids of strains BC31-1-A7 and BC31-3-A3 were identified as one phosphatidylethanolamine, some unidentified polar lipids and one aminolipid. Based on their distinct taxonomic characteristics, strains BC31-1-A7 and BC31-3-A3 represent two novel species in the genus . The names proposed to accommodate these two strains are sp. nov. and sp. nov., and the type strains are BC31-1-A7 (=MCCC M23246=KCTC 82569) and BC31-3-A3 (=MCCC M23216=KCTC 82302), respectively.
从太平洋深海沉积物中富集的聚对苯二甲酸乙二醇酯降解细菌菌群中分离到两株革兰氏阴性、需氧、棒状、类胡萝卜素着色、无鞭毛的新型细菌，分别命名为 BC31-1-A7 和 BC31-3-A3。这两株菌的最佳生长温度为 28-32°C，最佳 pH 值为 7.5，最佳盐浓度为 3-4%（w/v）。16S rRNA 基因序列分析表明，菌株 BC31-1-A7 和 BC31-3-A3 与 JCM 11811、KCTC 22173、DSM 13258、DSM 25030、JCM 33902 和 KCTC 72200 具有 96.8-98.9%的序列相似性。菌株 BC31-1-A7 和 BC31-3-A3 之间的 16S rRNA 基因序列相似性为 97.5%。菌株 BC31-1-A7 和 BC31-3-A3 的基因组 G+C 含量分别为 42.1mol%和 41.6mol%。菌株 BC31-3-A3、BC31-1-A7 及其 6 株近缘模式菌株之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 77.6-84.3%和 20.5-27.9%。所有菌株均检测到menaquinone-6 作为主要的异戊二烯醌。它们的主要脂肪酸为 iso-C、iso-C G 和 iso-C 3-OH。菌株 BC31-1-A7 和 BC31-3-A3 的主要极性脂为一个磷脂酰乙醇胺、一些未鉴定的极性脂和一个氨基脂。基于其独特的分类学特征，菌株 BC31-1-A7 和 BC31-3-A3 代表属中的两个新种。建议采用的名称分别为 sp. nov. 和 sp. nov.，其模式菌株分别为 BC31-1-A7（=MCCC M23246=KCTC 82569）和 BC31-3-A3（=MCCC M23216=KCTC 82302）。
