Center for Bioinformatics, The University of Kansas, Lawrence, KS 66047, USA.
Curr Opin Struct Biol. 2013 Apr;23(2):198-205. doi: 10.1016/j.sbi.2012.12.003. Epub 2013 Jan 5.
Structural characterization of protein-protein interactions across the broad spectrum of scales is key to our understanding of life at the molecular level. Low-resolution approach to protein interactions is needed for modeling large interaction networks, given the significant level of uncertainties in large biomolecular systems and the high-throughput nature of the task. Since only a fraction of protein structures in interactome are determined experimentally, protein docking approaches are increasingly focusing on modeled proteins. Current rapid advancement of template-based modeling of protein-protein complexes is following a long standing trend in structure prediction of individual proteins. Protein-protein templates are already available for almost all interactions of structurally characterized proteins, and about one third of such templates are likely correct.
蛋白质-蛋白质相互作用的结构特征在广泛的尺度上是我们理解分子水平生命的关键。鉴于大生物分子系统中存在显著的不确定性和任务的高通量性质，需要采用低分辨率方法来模拟大的相互作用网络。由于相互作用组中只有一部分蛋白质结构是通过实验确定的，因此蛋白质对接方法越来越关注建模的蛋白质。目前，基于模板的蛋白质-蛋白质复合物建模的快速发展遵循了单个蛋白质结构预测的长期趋势。几乎所有结构特征的蛋白质相互作用都有蛋白质-蛋白质模板，其中约三分之一的模板可能是正确的。
