大鼠内视网膜氧提取分数。
Inner retinal oxygen extraction fraction in rat.
机构信息
Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, USA.
出版信息
Invest Ophthalmol Vis Sci. 2013 Jan 21;54(1):647-51. doi: 10.1167/iovs.12-11305.
PURPOSE
Oxygen extraction fraction (OEF), defined by the ratio of oxygen consumption to delivery, may be a useful parameter for assessing the retinal tissue status under impaired circulation. We report a method for measurement of inner retinal OEF in rats under normoxia and hypoxia based on vascular oxygen tension (PO(2)) imaging.
METHODS
Retinal vascular PO(2) measurements were obtained in 10 rats, using our previously developed optical section phosphorescence lifetime imaging system. Inner retinal OEF was derived from retinal vascular PO(2) measurements based on Fick's principle. Measurements of inner retinal OEF obtained under normoxia were compared between nasal and temporal retinal sectors and repeatability was determined. Inner retinal OEF measurements obtained under normoxia and hypoxia were compared.
RESULTS
Retinal vascular PO(2) and inner retinal OEF measurements were repeatable (ICC ≥ 0.83). Inner retinal OEF measurements at nasal and temporal retinal sectors were correlated (R = 0.71; P = 0.02; n = 10). Under hypoxia, both retinal arterial and venous PO(2) decreased significantly as compared with normoxia (P < 0.001; n = 10). Inner retinal OEF was 0.46 ± 0.13 under normoxia and increased significantly to 0.67 ± 0.16 under hypoxia (mean ± SD; P < 0.001; n = 10).
CONCLUSIONS
Inner retinal OEF is a promising quantitative biomarker for the adequacy of oxygen supply for metabolism under physiologically and pathologically altered conditions.
目的
氧摄取分数（OEF）定义为氧消耗与输送的比值，可能是评估循环受损下视网膜组织状态的有用参数。我们报告了一种在正常氧和缺氧条件下基于血管氧张力（PO 2 ）成像测量大鼠内层视网膜 OEF 的方法。
方法
使用我们之前开发的光学切片磷光寿命成像系统，在 10 只大鼠中进行了视网膜血管 PO 2 测量。根据 Fick 原理，从视网膜血管 PO 2 测量中得出内层视网膜 OEF。比较了正常氧下获得的鼻侧和颞侧视网膜区域的内层视网膜 OEF 测量值，并确定了重复性。比较了正常氧和缺氧下的内层视网膜 OEF 测量值。
结果
视网膜血管 PO 2 和内层视网膜 OEF 测量值具有可重复性（ICC ≥ 0.83）。鼻侧和颞侧视网膜区域的内层视网膜 OEF 测量值相关（R = 0.71；P = 0.02；n = 10）。与正常氧相比，缺氧时视网膜动脉和静脉 PO 2 均显著降低（P < 0.001；n = 10）。正常氧下内层视网膜 OEF 为 0.46 ± 0.13，缺氧时显著增加至 0.67 ± 0.16（平均值 ± 标准差；P < 0.001；n = 10）。
结论
内层视网膜 OEF 是一种有前途的定量生物标志物，可用于评估生理和病理条件下代谢所需氧气供应的充足性。
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