患有龋齿疾病的青少年唾液中的促炎细胞因子。
Pro-inflammatory cytokines in saliva of adolescents with dental caries disease.
作者信息
Gornowicz Agnieszka, Bielawska Anna, Bielawski Krzysztof, Grabowska Stanisława Zyta, Wójcicka Anna, Zalewska Magdalena, Maciorkowska Elżbieta
机构信息
Department of Biotechnology, Medical University, Bialystok, Poland.
出版信息
Ann Agric Environ Med. 2012;19(4):711-6.
INTRODUCTION AND OBJECTIVE
Dental caries is an inflammatory disease with multifactorial etiology. The presented study was conducted to test the hypothesis that the elevation of salivary cytokines - interleukin-6 (IL-6), interleukin-8 (IL-8) and tumour necrosis factor (TNF-a) is changed in dental caries patients. IL-6, IL-8 and TNF-a are particularly relevant to inflammation, one of the very first responses of the host to a pathological insult.
MATERIALS AND METHODS
Whole saliva from 26 patients with dental caries, as well as 10 healthy persons, was investigated for the presence of IL-6, IL-8 and TNF-a by enzyme immunoassay - ELISA.
RESULTS
The results showed that an elevation of IL-6, IL-8 and TNF-a in unstimulated whole saliva in subjects with dental caries, compared with controls, increased and was statistically significant in all cases (p <0.05). The study also show a positive correlation between TNF-a and IL-8.
CONCLUSIONS
These data suggest links between the production of tumour necrosis factor (TNF-a), interleukin-6 (IL-6), interleukin-8 (IL-8) in saliva and dental caries disease.
引言与目的
龋齿是一种病因多因素的炎症性疾病。本研究旨在验证龋齿患者唾液中细胞因子——白细胞介素-6（IL-6）、白细胞介素-8（IL-8）和肿瘤坏死因子（TNF-α）水平升高这一假设。IL-6、IL-8和TNF-α与炎症密切相关，而炎症是宿主对病理损伤最早的反应之一。
材料与方法
采用酶联免疫吸附测定法（ELISA）检测26例龋齿患者及10名健康人的全唾液中IL-6、IL-8和TNF-α的含量。
结果
结果显示，与对照组相比，龋齿患者非刺激性全唾液中IL-6、IL-8和TNF-α水平升高，且在所有病例中均具有统计学意义（p<0.05）。研究还表明TNF-α与IL-8之间存在正相关。
结论
这些数据表明唾液中肿瘤坏死因子（TNF-α）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）的产生与龋齿疾病之间存在联系。
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