Department of Oral and Maxillofacial Surgery, Dental Research Center, Shahid Beheshti Medical University, Tehran, Islamic Republic of Iran.
Oral Surg Oral Med Oral Pathol Oral Radiol. 2013 Feb;115(2):e7-15. doi: 10.1016/j.oooo.2011.09.034. Epub 2012 May 30.
This study aimed to assess NanoBone as a carrier construct for mesenchymal stem cells (MSCs) and platelet-rich growth factor (PRGF).
In the calvarial bone of 8 mature New Zealand White male rabbits, four 8-mm defects were created. Each defect received one of the following treatments: Group 1, 0.2 mg Nano-hydroxyapatite (HA) granule + 2 mL culture medium; Group 2, 0.2 mg Nano-HA + 1 mL autologous PRGF + 2 mL acellular culture medium; Group 3, 0.2 mg Nano-HA + 2 mL culture medium containing 100,000 autogenous MSCs; Group 4, 0.2 mg Nano-HA + 2 mL culture medium containing 100,000 autogenous MSCs + 1 mL autologous PRGF.
Histomorphometric analysis at 6 and 12 weeks demonstrated significantly higher bone formation in group 4 (29.45% and 44.55%, respectively) (P < .05). Bone formation in groups 1, 2, and 3 were as follows: 11.35% and 32.53%, 29.10% and 39.74%, and 25.82% and 39.11%, respectively.
NanoBone with MSCs and PRGF seems to be an effective combination for bone regeneration in a rabbit calvaria model.
本研究旨在评估纳米骨作为间充质干细胞（MSCs）和富含血小板的生长因子（PRGF）的载体构建物。
在 8 只成熟的新西兰白兔的颅骨骨中，创建了四个 8mm 的缺损。每个缺损接受以下治疗之一：组 1，0.2mg 纳米羟基磷灰石（HA）颗粒+2mL 培养基；组 2，0.2mg 纳米-HA+1mL 自体 PRGF+2mL 去细胞培养基；组 3，0.2mg 纳米-HA+2mL 含 100,000 个自体 MSC 的培养基；组 4，0.2mg 纳米-HA+2mL 含 100,000 个自体 MSC 和 1mL 自体 PRGF 的培养基。
6 周和 12 周的组织形态计量学分析显示，组 4 的骨形成明显更高（分别为 29.45%和 44.55%）（P<0.05）。组 1、2 和 3 的骨形成分别为：11.35%和 32.53%、29.10%和 39.74%、25.82%和 39.11%。
纳米骨与 MSCs 和 PRGF 似乎是一种有效的组合，可用于兔颅骨模型中的骨再生。
