China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, PR China.
Int J Syst Evol Microbiol. 2013 Aug;63(Pt 8):2819-2822. doi: 10.1099/ijs.0.046896-0. Epub 2013 Jan 11.
In this study, two psychrophilic bacterial strains were isolated from the China No. 1 glacier in Xinjiang, north-west China. Cells were Gram-positive rods. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains belonged to the genus Cryobacterium. Phylogenetic analysis showed that they clustered together and are most closely related to Cryobacterium luteum CGMCC 1.11210(T), Cryobacterium flavum CGMCC 1.11215(T), Cryobacterium psychrophilum CGMCC 1.4292(T), Cryobacterium psychrotolerans CGMCC 1.5382(T) and Cryobacterium roopkundense CGMCC 1.10672(T). The major cellular fatty acids of the novel strains were anteiso-C15 : 0, anteiso-C15 : 1 A, iso-C16 : 0 and iso-C15 : 0. Both strains contained diphosphatidylglycerol, phosphatidylglycerol and one unidentified glycolipid in the cell membrane. The results of DNA-DNA hybridization and physiological tests allowed the genotypic and phenotypic differentiation of strains Hh34(T) and Hh28 from related species. However, their high DNA-DNA relatedness showed that they belong to the same novel species. Strain Hh34(T) (= NBRC 107883(T) = CGMCC 1.11211(T)) was selected as the type strain to represent this novel species, for which the name Cryobacterium levicorallinum sp. nov. is proposed.
在本研究中，从中国新疆的一号冰川中分离出了两株嗜冷细菌。细胞呈革兰氏阳性杆菌。基于 16S rRNA 基因序列的系统发育分析表明，这些菌株属于 Cryobacterium 属。系统发育分析表明，它们聚在一起，与 Cryobacterium luteum CGMCC 1.11210(T)、Cryobacterium flavum CGMCC 1.11215(T)、Cryobacterium psychrophilum CGMCC 1.4292(T)、Cryobacterium psychrotolerans CGMCC 1.5382(T)和 Cryobacterium roopkundense CGMCC 1.10672(T)最为密切相关。新菌株的主要细胞脂肪酸为 anteiso-C15 : 0、anteiso-C15 : 1 A、iso-C16 : 0 和 iso-C15 : 0。两株菌的细胞膜均含有双磷脂酰甘油、磷脂酰甘油和一种未知的糖脂。DNA-DNA 杂交和生理试验的结果允许菌株 Hh34(T)和 Hh28 与相关种在基因型和表型上的分化。然而，它们之间的高 DNA-DNA 同源性表明它们属于同一新种。菌株 Hh34(T) (= NBRC 107883(T) = CGMCC 1.11211(T))被选为代表该新种的模式株，该新种被命名为 Cryobacterium levicorallinum sp. nov.。
