China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100093, PR China.
Int J Syst Evol Microbiol. 2012 Jun;62(Pt 6):1296-1299. doi: 10.1099/ijs.0.033738-0. Epub 2011 Jul 22.
Gram-positive, rod-shaped bacteria, strains Hh8(T), Hh15(T) and Hh40-2, were isolated from the No. 1 glacier in Xinjiang, north-west China. Colonies of strain Hh8(T) were orange-yellow, convex and round on PYG plates. Strain Hh8(T) grew at 0-19 °C and pH 5.5-10.5. Colonies of strain Hh15(T), which was able to grow at 0-20 °C and pH 5.5-12, were lemon yellow, convex and round on PYG plates. Phylogenetic analysis based on 16S rRNA gene sequences showed that these three strains were related to members of the genus Cryobacterium. The major cellular fatty acids of the novel strains were anteiso-C(15:0), iso-C(16:0), iso-C(15:0) and anteiso-C(15:1) A. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, two novel species, Cryobacterium flavum sp. nov. (type strain Hh8(T) = CGMCC 1.11215(T) = NBRC 107879(T)) and Cryobacterium luteum sp. nov. (type strain Hh15(T) = CGMCC 1.11210(T) = NBRC 107880(T)), are proposed.
革兰氏阳性，杆状细菌，菌株 Hh8(T)、Hh15(T) 和 Hh40-2，从中国西北部新疆的 1 号冰川中分离得到。菌株 Hh8(T)的菌落呈橙黄色，在 PYG 平板上呈凸起的圆形。菌株 Hh8(T)在 0-19°C 和 pH5.5-10.5 条件下生长。菌株 Hh15(T)的菌落柠檬黄色，在 PYG 平板上呈凸起的圆形，能够在 0-20°C 和 pH5.5-12 条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，这三个菌株与 Cryobacterium 属的成员有关。这两个新菌株的主要细胞脂肪酸为 anteiso-C(15:0)、iso-C(16:0)、iso-C(15:0)和 anteiso-C(15:1)A。根据表型特征、系统发育分析和 DNA-DNA 同源性数据，提出了两个新种，即 Cryobacterium flavum sp. nov.（模式菌株 Hh8(T)=CGMCC 1.11215(T)=NBRC 107879(T)）和 Cryobacterium luteum sp. nov.（模式菌株 Hh15(T)=CGMCC 1.11210(T)=NBRC 107880(T)）。
